NAC对大鼠脑缺血再灌注引起胃黏膜损伤的影响

目的研究N-乙酰-L-半胱氨酸（N-acetylcysteine,NAC）对大鼠脑缺血再灌注引起应激性胃损伤的影响。方法采用四动脉结扎法建立大鼠全脑缺血模型,缺血后静脉注射NAC（150 mg/kg）,取胃后计数胃黏膜损伤指数（gastric mucosal damage index,GMDI）,测定胃黏膜组织中丙二醛（malonaldehyde,MDA）含量、超氧化物歧化酶（superoxide dismutase,SOD）活性,采用原位检测（TUNEL）法检测胃黏膜细胞凋亡情况。结果 NAC可以降低大鼠脑缺血再灌注引起应激性胃损伤时的胃黏膜损伤指数,使胃黏膜组织中MDA含量减少、SOD活性增强,抑制胃黏膜细胞凋亡。结论 NAC对大鼠脑缺血再灌注引起的应激性胃损伤具有保护作用,这种保护作用是通过抑制氧化应激、减少胃黏膜细胞凋亡而实现的。

Eeffect of N-acetylcysteine on brain ischemia/reperfusion-induced gastric mucosa injury in rats

Objective To investigate the effect of antioxidant N-acetylcysteine（NAC） on brain ischemia /reperfusion-induced stress gastric mucosa injury in rats.Methods Rat models of brain ischemia/reperfusion were established by the four vessel occlusion method.Following brain ischemia,NAC（150 mg/kg） was injected into the femoral vein.The rats were finally sacrificed to investigate gastric mucosal damage index（GMDI） and determine the content of malondialdehyde（MDA） and the activity of superoxide dismutase（SOD） in gastric mucosa.The apoptosis of gastric mucosal cells was detected by TUNEL.Results Injection of NAC into the femoral vein could decrease gastric mucosal damage index in stress gastric injury,reduce the content of MDA and increase the activity of SOD in the gastric mucosa,and inhibit gastric mucosal cellular apoptosis following brain ischemia/reperfusion.Conclusion NAC could protect against brain ischemia/reperfusion-induced stress gastric injury by the inhibition of oxidative stress and redction of cell apoptosis in gastric mucosa.