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An evaluation of an assay for DNA synthesis in lymphocytes with [H]thymidine and harvesting on to glass fibre filter discs |
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| Authors: | Theresa Ahern Geoffrey A. Taylor Colin J. Sanderson |
| Affiliation: | Division of Surgical Sciences, Clinical Research Centre, Watford Road, Harrow, HA1 3UJ, Middlesex, England |
| Abstract: | Different grades of glass fibre filter paper have been compared for harvesting cells labelled with tritium using a semi-automatic harvester. Grade GF/C (Whatman Ltd.) was selected for routine use although GF/B was found to be useful for whole blood cultures. The factors affecting the choice of thymidine concentration and activity are discussed and 1 μCi of [3H]thymidine (5 Ci/mMol)/0.2 ml culture is shown to give a linear relationship with total DNA synthesis for up to 22 h. |
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