Leptin对人成骨细胞增殖和功能的影响

目的 观察瘦素（leptin)对人成骨细胞增殖和功能的影响。方法 培养人成骨细胞，并观察其形态及功能变化。采用MTT比色法检测不同浓度的Leptin对人成骨细胞增殖的影响，同时观察不同浓度Letptin条件下骨钙素浓度的变化，并用细胞总蛋白较正。结果 体外培养的人成骨细胞大多呈长梭型，碱性磷酸酶组织化学染色、骨钙素免疫荧光组织化学染色阳性，培养液中加入β-甘油磷酸及L－抗坏血酸后钙茜素红染色阳性，在加入不同浓度Leptin后1、2、3天，人成骨细胞增殖无显著变化。Leptin可刺激骨钙素分泌，呈浓度依赖性（P＜0．05），但无时间依赖性（P＞0．05）。结论 Leptin对人成骨细胞增殖无影响，但可刺激人成骨细胞功能。

The effects of leptin on proliferation and function of human osteoblast]

OBJECTIVE: To observe the effect of leptin on osteoblast. METHODS: Human osteoblast primary culture was carried out, and the morphology and function of osteoblast were observed. The effects of different levels of leptin on osteoblast in different days were assessed by MTT colorimetry. Osteocalcin production was measured also. RESULTS: Human osteoblasts were fusiform in shape and were positive for alkaline phosphatase by histochemical staining, positive for osteocalcin by immunofluorescence staining, and positive by Alizarin Reds staining after mineralized upon supplementation with ascorbate and beta-glycerophosphate. On the first, second and third days, the proliferation of osteoblast, cultured with different concentrations of leptin, had no changes. The leptin-stimulated synthesis of osteocalcin of cells was found to be dose-dependent (P < 0.05), but not time-dependent (P > 0.05). CONCLUSION: The above data indicated that there were no evidences for the effects of leptin on the proliferation of human osteoblast, but leptin could enhance the function of human osteoblast.