添加载银纳米二氧化钛光亮漆体外细胞毒性及其抗菌性的实验研究

目的 研究添加载银纳米二氧化钛（Ag/TiO₂）抗菌剂对软衬硅橡胶光亮漆体外细胞毒性及抗菌性能的影响。方法 制备直径10 mm、厚度2 mm 的Silagum-Comfort软衬硅橡胶圆形试样，随机分成6组，将Ag/TiO₂按0、0.5％、1.0％、1.5％、2.0％、2.5%的重量比分别添加于光亮漆的基质和催化剂中，均匀搅拌后分别涂布于对应组试件表面一遍，制成含不同浓度抗菌剂的软衬硅橡胶试件；采用噻唑蓝比色法检测软衬硅橡胶试件对3T3细胞（小鼠胚胎成纤维细胞）的体外细胞毒性；使用粗糙度仪测量软衬硅橡胶试件表面粗糙度值（Ra）；采用贴膜法检测软衬硅橡胶试件的体外抗白色假丝酵母菌性能。结果 各实验组细胞毒性级别为0~2级。光亮漆的Ra值随着抗菌剂浓度的增加有所提高，但各浓度抗菌剂组与0组相比无统计学差异。随着抗菌剂浓度的增加，抗菌率也提高；抗菌剂浓度为2.0%和2.5%时，抗菌率已达到 97.5%和96.5%。结论 Ag/TiO₂光亮漆具有良好的抗菌效果，能有效改善软衬硅橡胶的抗菌性能。

Cytotoxicity and antibacterial effect of silicone-based soft liner varnish containing Ag/TiO2:an in vitro study

Objective We aim to evaluate the cytotoxicity and antibacterial effects of Silagum-Comfort (SLC) siliconebased soft liner varnish containing Ag/TiO₂in vitro. Methods Silicone-based liner circular specimens, with a diameter of 10 mm and a thickness of 2 mm, were created and divided into six groups randomly. Ag/TiO₂was incorporated into the SLC varnish based on the agent/material weight rate of 0, 0.5%, 1.0%, 1.5%, 2.0%, and 2.5%. The mixed varnish was used to polish the SLC specimens. Antibacterial specimens (ATSLC) were created. Methyl thiazolyl diphenyl-tetrazolium bromide assay method was used to evaluate the 3T3 cell cytotoxicity of ATSLC in vitro. Surface roughness (Ra) measurements of ATSLC were obtained. The antibacterial effect of ATSLC on Candida albicans was examined using the pelliclesticking method. Results The cytotoxicity of ATSLC was graded from 0 to 2. The Ra of ATSLC increased when Ag/TiO₂agent concentration increased. However, the differences were not statistically significant compared with the 0 group. With increasing Ag/ TiO₂concentration, the antibacterial agent concentration and the antibacterial rate of ATSLC also increased. When the Ag/ TiO₂ antibacterial agent concentration reached 2.0% and 2.5%, the antibacterial rate increased to 97.5% and 96.5, respectively. Conclusion Varnish containing Ag/TiO₂has excellent antibacterial effect and can significantly improve the antibacterial effect of silicone-based soft liner.