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| 反相高效液相色谱法测定茶色素注射液中没食子酸的含量 | |
| 引用本文： | 吕慧怡,韩国柱,李楠,杨美燕. 反相高效液相色谱法测定茶色素注射液中没食子酸的含量[J]. 中国药房, 2005, 16(21): 1657-1658 |
| 作者姓名： | 吕慧怡 韩国柱 李楠 杨美燕 |
| 作者单位： | 1. 大连医科大学附属第二医院药剂科,大连市,116027 2. 大连医科大学药理教研室,大连市,116023 3. 大连理工大学化学系,大连市,116023 |
| 摘 要： | 目的:建立茶色素注射液中没食子酸含量的测定方法。方法:采用反相高效液相色谱法,色谱柱为C18,流动相为乙酸-甲 醇-二甲基甲酰胺-水(2:8:30:160),检测波长为280nm,流速为0．5ml／min,柱温为20℃。结果:没食子酸检测浓度在8．4- 92．4μg／ml范围内线性关系良好(r=0．9 997),平均加样回收率为99．13％(RSD=1．55％)。结论:本方法特异性、灵敏度、精密 度、回收率高,重现性好,可为茶色素注射液质量标准的制订提供依据。 |
| 关 键 词： | 茶色素注射液 反相高效液相色谱法 没食子酸 含量 |
| 文章编号： | 1001-0408（2005）21-1657-02 |
| 收稿时间： | 2004-11-22 |
| 修稿时间： | 2005-01-10 |
Content Determination of Gallic Acid in Tea Pigment Injection by RP- HPLC |
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| LV Huiyi,HAN Guozhu,LI Nan,YANG Meiyan. Content Determination of Gallic Acid in Tea Pigment Injection by RP- HPLC[J]. China Pharmacy, 2005, 16(21): 1657-1658 | |
| Authors: | LV Huiyi HAN Guozhu LI Nan YANG Meiyan |
| Affiliation: | 1,Dept .of Pharmacy, The Second Affiliated Hospital of Dalian Medical University, Dalian 116027,China;2 School of Pharmaceutical Science,Dalian Medical University,Dalian l16023,China;3, Dept.of Chemistry, l |
| Abstract: | OBJECTIVE: To develop a method for the determination of gallic acid in tea pigment injection. METHODS: RP HLPC method was adopted in which the separation of gallic acid was performed on C18 chromatographic column with a column temperature of 20℃; the mobile phase was composed of acetic acid - McOH - DMF - H2O(2 : 8 : 30 : 160) with a flow rate of 0.5ml/ mm and detection wavelength of 280nm .RESULTS: The linear range of gallic acid was 8.4-92.4μg/ ml( r = 0.9 997),the average recovery rate was 99.13% (RSD = 1.55%) .CONCLUSION: The method developed in the present study is of high specificity .sensitivity .precision,recovery and reproducibility and it can provide a basis for the formulation of quality control method for tea pigment injection. |
| Keywords: | Tea pigment injection RP - HLPC Gallic acid Content |
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