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A new method for isolation and differentiation of native Entamoeba histolytica and E. dispar cysts from fecal samples |
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| Authors: | B Walderich L Müller R Bracha J Knobloch G D Burchard |
| Institution: | Institut für Tropenmedizin, Forschungslaboratorien, Wilhelmstrasse 27, D-72074 Tübingen, Germany Tel.: +49/7071/2982929; Fax: +49/7071/293017; E-mail: brigitte.walderich@uni-tuebingen.de, DE Weizmann Institute of Science, Rehovot 76100, Israel, IL Bernhard Nocht Institut, Bernhard-Nocht-Stra?sse 74, D-20359 Hamburg, Germany, DE |
| Abstract: | A new method for the purification of protozoan cysts from feces was established, allowing the isolation of native cysts. The procedure consists of two sucrose-density gradients and enzymatic digestion of cellulose particles by cellulase and can be accomplished in a few hours. The cyst fractions were differentiated into Entamoeba histolytica and E. dispar using the DNA probes P145 and B133 in a dot-blot test. Received: 7 March 1997 / Accepted: 24 March 1997 |
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