7种吸收促进剂的鼻黏膜毒性及评价方法

目的:考察常用吸收促进剂的鼻黏膜毒性.方法:采用测定黏膜纤毛持续运动时间、测定黏膜纤毛输送速率和考察黏膜形态3种方法评价了7种[十二烷基硫酸钠(SDS),去氧胆酸钠(SDC),苄泽35(Brij35),吐温80(Tween 80),乙二胺四乙酸钠(EDTANa2),2-羟丙基-β-环糊精(2-Hp-β-CD),卵磷酯(lecithin)]常用的吸收促进剂的鼻黏膜毒性.结果:对纤毛持续运动时间的影响1%SDS>1%SDC>1%Brij35>5%Tween80>0.1?TA>5%HP-β-CD>1%lecithin.对纤毛输送速率的影响1%Brij35>1%SDC>1%SDS>0.1?TA>1%lecithin>5%Tween80>5%HP-β-CD.黏膜形态学考察毒性1%SDS≈1%SDC≈1%Brij35>5%Tween80>0.1?TA≈5%HP-β-CD≈1%lecithin.以上浓度均为质量分数.结论:3种评价鼻黏膜毒性方法之间具有很好的相关性.

Influence of seven absorption enhancers on nasal mucosa--assessment of toxicity

OBJECTIVE: To study the influence of seven different absorption enhancers on nasal mucosa. METHODS: By testing last time of ciliary movement, velocity of ciliary movement, ciliary structural and specific cellular changes of nasal mucosa the influence of seven different absorption enhancers on nasal mucosa. RESULTS: The effect on lasting time of ciliary movement was 1%SDS>1%SDC>1%Brij35>5%Tween80>0.1%EDTA>5%HP-beta-CD>1%lecithin the effect on velocity of ciliary movement 1%Brij35>1%SDC>1%SDS>0.1%EDTA>1%lecithin>5%Tween80>5%HP-beta-CD,and the effect on ciliary structural and specific cellular changes of nasal mucosa 1%SDS approximately 1%SDC approximately 1%Brij35>5%Tween80>0.1%EDTA approximately 5% HP-beta-CD approximately 1%lecithin. CONCLUSION: The three methods have good correlation.