TrkA基因在神经母细胞瘤增殖及形态分化中的作用

目的 观察酪氨酸激酶受体A(TrkA)基因抑制神经母细胞瘤生长、诱导其分化的可行性。方法 利用脂质体转染法将TrkA cDNA重组质粒转染入人神经母细胞瘤(NB)SH-SY5Y细胞系中,应用RT-PCR技术鉴定转染后基因的表达,比较转染前后SH-SY5Y细胞的增殖率及细胞的分化程度。结果 成功转染TrkA基因并在SH-SY5Y细胞中稳定表达,转染后SH-SY5Y细胞的增殖率较亲代细胞明显下降(P<0.01),细胞的分化程度增加(P<0.01)。结论 TrkA基因的高表达能有效抑制神经母细胞瘤的生长,并能促其向良性分化,为神经母细胞瘤的基因治疗提供理论依据。

The role of TrkA gene in the proliferation and differentiation of neuroblastoma

Objective To investigate the role of TrkA gene in the proliferation and differentiation ofneuroblastoma. Methods Recombined TrkA cDNA plasmid was transferred into neuroblastoma cells (SH-SY5Ycell line) by lipofect method. The expression of TrkA gene of cells before and after transfection wasdetected by RT-PCR. The proliferation rate and differentiation extent of parent SH-SY5Y cells were com-pared with TrkA-transfectant SH-SY5Y cells. Results TrkA gene was successfully transferred into SH-SY5Y cells and can be stably expressed. The capability of proliferation of the transferred cells was obviouslydecreased (P<0.01), the differentiation extent of the transferred cells was increased (P<0.01).Conclu-sions The proliferation of neuroblastoma is effectively inhibited by transferred TrkA gene. The TrkA-transferred SY5Y cells can produce a tendency to benign differentiation of neuroblastoma.