葡萄籽原花青素对人皮肤鳞状细胞癌A431细胞增殖和凋亡的影响

目的:明确葡萄籽原花青素(GSP)对人皮肤鳞状细胞癌A431细胞增殖和凋亡的影响.方法:体外培养A431细胞,用不同浓度的GSP(5、10、20、40、80μg/mL)处理,采用MTT实验和克隆形成实验检测细胞活力和增殖能力,Western blot检测细胞通路PI3K/Akt/GSK-3β信号转导通路蛋白的表达.结果...

Effect of grape seed proanthocyanidin on proliferation and apoptosis of human skin squamous cell carcinoma A431 cells

Objective: To determine the effect of grape seed proanthocyanidins (GSP) on proliferation and apoptosis of human skin squamous cell carcinoma A431 cells. Methods: A431 cells were cultured in vitro and treated with different concentrations of GSP (5, 10, 20, 40, 80 μg/mL). The cell viability and proliferation were detected by MTT assay and colony formation assay respectively. The protein level of PI3K/Akt/GSK-3β signal transduction pathway was detected by Western blot. Results: As the increasing of GSP concentration, the cell viability and cell clone level of A431 decreased gradually, apoptosis rate increased. The protein level of p-PI3K, p-Akt, p-GSK-3β decreased after treated with GSP. Conclusion: GSP can induced apoptosis and inhabited proliferation of A431 cells, which may be related with the activation of PI3K/Akt/GSK-3β signaling pathway.