HMGB1蛋白在口腔鳞癌组织中的表达及其与患者预后的关系

目的:探讨口腔鳞癌(OSCC)组织中高迁移率族蛋白1(HMGB1)蛋白的表达情况，并分析其与临床病理特征及预后的关系。方法:采用免疫组织化学SP法分别检测HMGB1在126例OSCC组织和30例正常口腔黏膜组织中的表达；分析HMGB1表达情况与OSCC临床病理特征的关系；Kaplan-Meier法分析HMGB1蛋白表达与术后总生存期(OS)的关系，Cox模型多因素预后分析。结果:OSCC组HMGB1蛋白高表达率高于对照组(P＜0.05)；OSCC组织中HMGB1蛋白高表达与肿瘤分化程度、临床分期、TNM分期和淋巴结转移有关(P＜0.05)；生存分析显示，HMGB1高表达组患者5年生存率低于HMGB1低或无表达组患者(P＜0.05)；单因素分析显示，HMGB1蛋白、临床分期、TNM分期及淋巴结转移为影响OSCC预后的因素(P＜0.05)；Cox多因素分析显示，HMGB1是影响OSCC患者预后的独立因素(P＜0.05)。结论:HMGB1蛋白在OSCC组织中高表达，与患者的肿瘤分化程度、临床分期、TNM分期和淋巴结转移等病理特征有关，并显著影响预后。

The relationship between the expression of HMGB1 protein and prognosis in oral squamous cell carcinoma

Objective:To explore the expression of high mobility group box-1(HMGB1) in oral squamous cell carcinoma (OSCC) tissues and to analyze its relationship with the clinicopathological features and prognosis.Methods:Immunohistochemical SP method was used to detect the expression of HMGB1 in 126 OSCC tissues and 30 normal oral mucosa tissues,respectively.The relationship between HMGB1 expression and clinicopathological features of OSCC was analyzed.Kaplan-Meier method was used to analyze the relationship between HMGB1 protein expression and overall survival (OS),and Cox model was used to analyze multivariate prognosis.Results:The high positive expression rate of HMGB1 in OSCC group was higher than that in control group (P＜0.05).The high expression of HMGB1 protein in OSCC was related to the degree of differentiation,clinical stage,TNM stage and lymph node metastasis (P＜0.05).Survival analysis showed that the 5-year survival rate of patients with high expression of HMGB1 was lower than that of patients with low or no expression of HMGB1 (P＜0.05).Univariate analysis showed that HMGB1 protein,clinical stage,TNM stage and lymph node metastasis were the prognostic factors of OSCC (P＜0.05).Cox multivariate analysis showed that HMGB1 was an independent prognostic factor in OSCC patients (P＜0.05).Conclusion:HMGB1 protein is highly expressed in OSCC tissues,which is associated with degree of differentiation,clinical stage,TNM stage,lymph node metastasis and other pathological characteristics,and significantly affects the prognosis.