牙龈卟啉单胞菌脂多糖促进食管鳞癌细胞的增殖和迁移

目的:评估牙龈卟啉单胞菌脂多糖（Pg-LPS）对食管鳞癌细胞增殖、迁移能力的影响，探索其可能的机制。方法:培养Het-1A和EC109细胞，实验组加入不同浓度Pg-LPS，对照组加入等量培养基。分别采用CCK-8、Transwell迁移实验或细胞划痕实验检测两组细胞增殖、迁移能力的变化；qRT-PCR、Western blot技术检测增殖、迁移相关基因表达量的改变。结果:Het-1A细胞经Pg-LPS作用24 h、48 h后，其光密度值较对照组显著上升。EC109细胞在5 μg/mL及10 μg/mL的Pg-LPS作用后24 h、48 h与对照组相比光密度值上升；1 μg/mL的Pg-LPS作用24 h后光密度值较对照组上升，差异有统计学意义（P<0.05），而作用48 h后，无明显统计学差异（P>0.05）。Pg-LPS作用后，Het-1A细胞穿膜数量多于对照组，EC109细胞相对覆盖面积高于对照组。Pg-LPS作用后Het-1A和EC109细胞增殖、迁移相关基因mRNA及ARTN蛋白表达量较对照组明显升高。结论:Pg-LPS可以促进Het-1A和EC109细胞的增殖、迁移；Pg-LPS作用于细胞后ARTN的表达量增加，进一步促进AKT1、CCND1、MTA1、CXCR4表达，可能是Pg-LPS影响食管鳞癌细胞增殖和迁移的机制之一。

Porphyromonas gingivalis lipopolysaccharide promotes the proliferation and migration of esophageal squamous cell carcinoma cells

Objective:To evaluate the effect of porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on the proliferation and migration of esophageal squamous cell carcinoma cells，and explore its possible mechanisms.Methods:The Het-1A and EC109 cells were cultured in vitro.In the experimental group，Het-1A and EC109 cells were cultured in the presence of Pg-LPS，while Het-1A and EC109 cells in the control group were treated with the same amount of medium.The proliferation and migration of Het-1A and EC109 cells in the control group or Pg-LPS treated group were detected by CCK-8，Transwell or wound healing assays.qRT-PCR and Western blot were used to evaluate the changes of gene expression levels related to proliferation and migration.Results:The optical density values of Het-1A cells treated with Pg-LPS for 24 or 48 hours were significantly higher than that of the control group.The optical density values of EC109 cells after treated with 5 μg/mL or 10 μg/mL Pg-LPS for 24 or 48 hours were significantly higher than that of the control group.After treatment with 1 μg/mL Pg-LPS for 24 h，the optical density values of EC109 cells increased compared with the control group，the difference was statistically significant(P<0.05)，but there was no significant difference after 48 hours(P>0.05).The number of Het-1A cells penetrating membrane was significantly higher than that of the control group after treated with Pg-LPS.The relative coverage area of EC109 cells was significantly higher than that of the control group after treated with Pg-LPS for 24 hours or 48 hours.The mRNA expression of genes related to proliferation and migration and the expression level of ARTN protein in Het-1A and EC109 cells treated with Pg-LPS were significantly higher than those in the control group.Conclusion:Pg-LPS could promote the proliferation and migration of Het-1A and EC109 cells.The expression level of ARTN increased after treated with Pg-LPS，and further promoted the expression of AKT1，CCND1，MTA1 and CXCR4，which may be one of the mechanisms of Pg-LPS affecting the proliferation and migration of esophageal squamous cell carcinoma cells.