贵州省2017-2018年非伤寒沙门菌临床分离株耐药及分子分型研究

目的 了解贵州省2017-2018年非伤寒沙门菌(NTS)的血清型分布、耐药情况与脉冲凝胶电泳(PFGE)分子分型特征.方法 对分离自贵州省临床病例的191株非伤寒沙门菌菌株进行生化鉴定、血清型分型,微量肉汤稀释法测定16种抗生素的最小抑菌浓度,PFGE进行分子分型分析.结果 191株非伤寒沙门菌分出18种血清型,鼠伤...

Antimicrobial resistance and molecular typing of non-typhoid Salmonella clinical isolates from Guizhou Province in 2017-2018

To understand the serotype distribution, antimicrobial resistance and pulsed field gel electrophoresis (PFGE) molecular typing characteristics of non-typhoid salmonella (NTS) in Guizhou Province, we identified 191 NTS clinical isolates through biochemical methods and performed serotyping with the micro broth dilution method against 16 antimicrobial agents; molecular typing was detected by PFGE. A total of 191 clinical isolates were identified as 18 serotypes. The dominant serotypes were Salmonella Typhimurium (45.5%, 87/191) and Salmonella Enteritidis (29.3%, 56/191). A total of 96.8% (185/191) of the isolates showed resistance to the antimicrobial agents tested. Resistance to sulfaisoxazole, streptomycin, ampicillin, tetracycline and doxycycline reached as high as >60.0%. Resistance to ceftriaxone and ciprofloxacin was found in 7.9% (15/191) and 13.1% (25/191) of the isolates, respectively. Approximately 84.3% (161/191) of the isolates were identified as multi-drug resistant (MDR). Sixty-nine antimicrobial resistance profiles were observed, and the major antimicrobial resistance profiles were ampicillin+streptomycin+sulfamethoxazole+nalidixic, and ampicillin+chloramphenicol+ streptomycin+sulfamethoxazole+ tetracycline+doxycycline+trimethoprim/sulfamethoxazole. Eighty-seven S.Typhimurium isolates were divided into 60 PFGE types, and the dominant type was JPXX01.GZ0047 (12.6%, 11/87). Fifty-six S. Enteritidis isolates were divided into 21 PFGE types, and the dominant type was JEGX01.GZ0005 (23.3%, 13/56). S. Typhimurium and S. Enteritidis were the dominant serotypes of NTS isolates in Guizhou Province. The prevalence of MDR is currently concerningly high. The PFGE types of NTS isolates were highly genetic diverse, and some types were related to antimicrobial resistance profiles.