Septin9甲基化及膳食因素对结直肠癌发病的影响

目的:探讨Septin9甲基化与膳食因素对结直肠癌(colorectal cancer，CRC)发病的影响。方法:采用病例-对照研究分析，收集CRC病例60例，根据年龄进行1∶1匹配收集健康人群60例作为对照组。采用χ2检验比较病例和对照组的基本特征、膳食和Septin9甲基化分布，采用非条件Logistic回归分析比较不同的Septin9基因甲基化和膳食对CRC发病的影响。结果:病例组和对照组的年龄、性别、民族、家庭人均月收入、文化程度和一级亲属肿瘤史，均无显著统计学差异（P＞0.05）；病例组中Septin9基因甲基化阳性24例（40.0%），显著高于对照组（P＜0.05），但病例组和对照组的平均每日蔬菜摄入量和家畜肉类摄入量无显著统计学差异（P＞0.05）；通过单因素分析所有变量，并将有统计学意义的变量纳入多因素逻辑回归中，最后确定CRC的独立危险因素包括Septin9甲基化（OR=1.88，95%CI:1.56~2.27）和平均家畜肉摄入量≥300 g/d（OR=1.30，95%CI:1.24~1.37）；以Septin9甲基化阳性且平均蔬菜摄入量<400 g/d（OR=4.650，95%CI:1.393~15.518）为参照组，Septin9甲基化阳性且平均蔬菜摄入量≥400 g/d显著降低CRC的发病风险（OR=4.650，95%CI:1.393~15.518）；以Septin9甲基化阳性且平均家畜肉摄入量<300 g/d（OR=4.500，95%CI:1.463~13.843）为参照组，Septin9甲基化阳性且平均家畜肉摄入量≥300 g/d显著增加CRC的发病风险（OR=7.333，95%CI:2.195~24.501）。结论:Septin9甲基化阳性人群蔬菜摄入量≥400 g/d降低CRC的发病风险，Septin9甲基化阳性且平均家畜肉摄入量≥300 g/d显著增加CRC的发病风险，对此人群减少脂肪肉类摄入并增加蔬菜摄入量具有降低CRC发病风险的可能。

Effect of Septin9 methylation and dietary factors on colorectal cancer

Objective:To investigate the effect of Septin9 methylation and dietary factors on colorectal cancer.Methods:Case-control study analysis was used.60 cases of colorectal cancer patients and 60 healthy people were collected as control group according to the age matching.χ2 test were used to compare the basic characteristics,dietary factors and Septin9 methylation distribution of the case and control groups.Unconditional Logistic regression analysis was used to compare the effects of different combination of Septin9 gene methylation and dietary interaction on the incidence of colorectal cancer.Results:There were no statistically significant differences between the case group and the control group in age,gender,ethnicity,family per capita monthly income,educational level and first-degree relative tumor history (P＞0.05).In the case group,24 cases (40.0%) were positive for Septin9 methylation,which was significantly higher than that in the control group (P＜0.05).However,there was no statistically significant difference in the average daily vegetable intake and livestock meat intake between the case group and the control group (P＞0.05).All variables were analyzed by univariate analysis,and the variables with statistical significance were included in multivariate Logistic regression.The independent risk factors of CRC were identified as Septin9 methylation (OR=1.88,95%CI:1.56~2.27) and average livestock meat intake ≥300 g/d (OR=1.30,95%CI:1.24~1.37).Compared with Septin9 methylation positive and average vegetable intake (≥400 g/d) group,Septin9 methylation positive and average vegetable intake (<400 g/d) significantly increased the risk of colorectal cancer (OR=4.650,95%CI:1.393~15.518).Compared with Septin9 methylation positive and average meat intake （＜300 g/d group）.Septin9 methylation positive and average meat intake （≥300 g/d） significantly increased the risk of colorectal cancer (OR=7.333,95%CI:2.195~24.501).Conclusion:The vegetable intake of Septin9 methylated positive people ≥400 g/d reduced the risk of CRC.Septin9 methylation positive and average livestock meat intake ≥300 g/d significantly increased the risk of CRC.Reducing the intake of fatty meat and increasing the intake of vegetables in this population may reduce the risk of CRC.