| 玻璃体内注射腺相关病毒介导原纤维蛋白-2基因干扰对小鼠视网膜的影响 |
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| 引用本文： | 张瑞雪,蒋文君,石永伟,丁捷,许静,温莹,毕宏生. 玻璃体内注射腺相关病毒介导原纤维蛋白-2基因干扰对小鼠视网膜的影响[J]. 眼科新进展, 2021, 0(9): 806-811. DOI: 10.13389/j.cnki.rao.2021.0169 |
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| 作者姓名： | 张瑞雪 蒋文君 石永伟 丁捷 许静 温莹 毕宏生 |
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| 作者单位： | 250014 山东省济南市，山东中医药大学（张瑞雪，石永伟，丁捷，许静）；250002 山东省济南市，山东中医药大学眼科研究所,山东省中西医结合眼病防治重点实验室（蒋文君，毕宏生）；250002 山东省济南市,山东中医药大学附属眼科医院（温莹，毕宏生） |
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| 摘 要： | 目的 探讨玻璃体内注射腺相关病毒(AAV)介导原纤维蛋白-2(FBN2)基因干扰进行基因敲低对小鼠视网膜的影响.方法 54只8周龄C57BL/6J小鼠随机分为3组:正常对照组、阴性对照组、AAV组.正常对照组小鼠正常饲养,阴性对照组和AAV组小鼠右眼玻璃体内分别注射3μL阴性病毒或AAV.于注射后2周、3周、4周利用共...
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| 关 键 词： | 腺相关病毒 原纤维蛋白-2 玻璃体内注射 视网膜病变 |
Effect of intervening the fibrillin-2 gene via intravitreal injection of adeno-associated virus on mouse retina |
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| ZHANG Ruixue1,JIANG Wenjun2,SHI Yongwei1,DING Jie1,XU Jing1,WEN Ying3,BI Hongsheng2,' target="_blank" rel="external">3. Effect of intervening the fibrillin-2 gene via intravitreal injection of adeno-associated virus on mouse retina[J]. Recent Advances in Ophthalmology, 2021, 0(9): 806-811. DOI: 10.13389/j.cnki.rao.2021.0169 |
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| Authors: | ZHANG Ruixue1,JIANG Wenjun2,SHI Yongwei1,DING Jie1,XU Jing1,WEN Ying3,BI Hongsheng2,' target=" _blank" rel=" external" >3 |
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| Affiliation: | 1.Shandong University of Traditional Chinese Medicine,Jinan 250014,Shandong Province,China2.Eye Institute of Shandong University of Traditional Chinese Medicine,Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases,Jinan 250002，Shandong Province,China3.Eye Hospital Affiliated to Shandong University of Traditional Chinese Medicine，Jinan 250002，Shandong Province,China |
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| Abstract: | Objective To investigate the effect of intervening the fibrillin-2 (FBN2) gene via intravitreal injection of adeno-associated virus (AAV) on mouse retina.Methods Fifty-four 8-week-old C57BL/6J mice were randomly divided into the normal control group, negative control group and AAV group, with 18 mice in each group. Mice in the normal control group were normally fed, while those in the latter two groups received an intravitreal injection of 3 μL of negative virus or AAV in the right eye, respectively. At 2, 3 and 4 weeks after injection, the changes of fundus and visual function, the thickness of outer nuclear layer (ONL) of retina were examined by the scanning laser ophthalmoscope (SLO), electroretinogram (ERG) and optical coherence tomography (OCT). In addition, mRNA and protein levels of FBN2 in mouse retina were detected by real-time quantitative PCR (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively.Results SLO results showed the presence of drusen-like deposits in mouse ocular fundus of AAV group after 2 weeks of intravitreal injection, which remained until the end of the experiment. OCT results showed irregular and punctate high-density reflex area in the retinal pigment epithelium of AAV group. After 2, 3 and 4 weeks of injection, the thickness of ONL in AAV group was significantly smaller than that of normal control group and negative control group (both P<0.05). ERG results showed that the amplitudes of Rod-b and Max-a in AAV group after 2, 3 and 4 weeks of injection were significantly lower than those of negative control group and normal control group (all P<0.05). RT-PCR and ELISA results showed that mRNA and protein levels of FBN2 in AAV group were significantly lower than those in negative control group and normal control group at 2, 3 and 4 weeks after injection (all P<0.05).Conclusion Intervene of FBN2 by intravitreal injection of AAV can induce retinopathy in mice. |
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| Keywords: | adeno-associated virus fibrillin-2 intravitreal injection retinopathy |
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