首页 | 本学科首页   官方微博 | 高级检索  
     

药物流产出血模型小鼠雌、孕激素水平与Th17/Treg亚群的关系#br#
引用本文:胡晓燕,郭桂芝,肖婷婷. 药物流产出血模型小鼠雌、孕激素水平与Th17/Treg亚群的关系#br#[J]. 天津医药, 2021, 49(5): 490-494. DOI: 10.11958/20202707
作者姓名:胡晓燕  郭桂芝  肖婷婷
作者单位:贵州医科大学附属医院
摘    要:摘要:目的 研究药物流产出血模型小鼠的雌、孕激素水平与Th17/Treg亚群的关系。方法 选取清洁级Balb/c雌性小鼠32只,通过随机数字表法分为正常妊娠组(A组)、小剂量米非司酮组(B组)、大剂量米非司酮组(C组)、米非司酮+宫清颗粒组(D组),每组8只。B、C、D组小鼠于妊娠第6天时分别使用米非司酮0.88 mg·kg-1·d-1、1.5 mg·kg-1·d-1及米非司酮(1.5 mg·kg-1·d-1)+宫清颗粒组(308.16 mg·kg-1·d-1)灌胃。采用改良碱性正铁血红素法检测子宫出血量;采用放射免疫法检测血清雌二醇(E2)、孕酮(P)水平;采用流式细胞术双染色法检测子宫组织Th17(CD3+CD4+IL-17A+)、Treg(CD4+CD25+Foxp3+)及Th17/Treg亚群比值;采用逆转录聚合酶链式反应(RT-PCR)法检测子宫组织白细胞介素(IL)-17A、IL-10、转化生长因子(TGF)-β mRNA水平。结果 B、C、D组子宫出血量依次减少。药物流产后,A、B、C、D组小鼠E2水平依次升高,而P水平依次降低。Th17亚群依次升高,Treg亚群依次降低,Th17/Treg亚群比值依次升高(均P<0.05)。IL-17A mRNA相对表达量依次升高,而IL-10、TGF-β mRNA相对表达量依次降低(均P<0.05)。血清E2与Th17、Th17/Treg和IL-17A水平呈正相关,与Treg、IL-10和TGF-β水平呈负相关;血清P与Treg、IL-10和TGF-β水平呈正相关,与Th17、Th17/Treg和IL-17A水平呈负相关(均P<0.05)。结论 药物流产后的异常子宫出血血清E2、P水平变化与Th17/Treg亚群比值变化关系密切,诱导Th17/Treg亚群向Th17偏移有助于减少子宫出血。

关 键 词:流产  人工;子宫出血;雌二醇;孕酮;Th17细胞;T淋巴细胞  调节性  
收稿时间:2020-09-29
修稿时间:2021-01-18

The relationship between estrogen and progesterone levels and Th17/Treg subgroups in the hemorrhagic model mice after medical abortion
HU Xiao-yan,GUO Gui-zhi,XIAO Ting-ting. The relationship between estrogen and progesterone levels and Th17/Treg subgroups in the hemorrhagic model mice after medical abortion[J]. Tianjin Medical Journal, 2021, 49(5): 490-494. DOI: 10.11958/20202707
Authors:HU Xiao-yan  GUO Gui-zhi  XIAO Ting-ting
Abstract:Abstract: Objective To study the relationship between estrogen (E2) and progesterone (P) levels and Th17/Treg subgroups in the hemorrhagic model mice after medical abortion. Methods Thirty-two female Balb/c mice with clean grade were selected and divided into normal pregnancy group (group A), low-dose mifepristone group (group B), high-dose mifepristone group (group C) and mifepristone + gongqing granules group (group D) by random number table method, with 8 mice in each group. Mice in group B, C and D were gavaged with mifepristone 0.88 mg·kg-1·d-1, 1.5 mg·kg-1·d-1 and mifepristone (1.5 mg·kg-1·d-1) + gongqinggranules (308.16 mg·kg-1·d-1) during d 6 pregnancy, respectively. Alkaline hematin photometric method was used to detect uterine blood loss. The serum E2 and P levels were detected by rdioimmunoassay (RIA). The uterus tissue Th17 (CD3+CD4+IL-17A+), Treg (CD4+CD25+Foxp3+) and the ratio of Th17/Treg subsets were detected by flow cytometry double staining method, and the uterus tissue interleukin 17A (IL-17A), interleukin 10 (IL-10) and transforming growth factor (TGF-β) mRNA levels were detected by RT-PCR method. Results The uterine bleeding volume decreased in turn in group B, group C and group D (P<0.05). After drug abortion, the E2 levels increased in turn in group A, group B, group C and group D, while the P levels decreased in turn in group A, group B, group C and group D (P<0.05). The ratio of Th17 and Th17/Treg subgroups of mice decreased in turn in the four groups, while the Treg subgroup ratio decreased in turn (P<0.05). The levels of IL-17A mRNA increased in turn in the four groups (P<0.05), andthe levels of IL-10 mRNA and TGF- β mRNA were decreased in turn in the four groups (P<0.05). The serum level of E2 was positively correlated with Th17, Th17/Treg and IL-17A mRNA, and negatively correlated with Treg, IL-10 and TGF-β (all P<0.05). The serum level of P was positively correlated with Treg, IL-10 and TGF-β, and negatively correlated with Th17, Th17/Treg and IL-17A (all P<0.05). Conclusion The changes of serum E2 and P levels in abnormal uterine bleeding after medical abortion are closely related to the changes of Th17/Treg subgroup ratio, and inducing Th17/Treg subgroup to migrate to Th17 can help reduce uterine bleeding.
Keywords:abortion   induced  uterine hemorrhage  estradiol  progesterone  Th17 cells  T-lymphocytes   regulatory  
点击此处可从《天津医药》浏览原始摘要信息
点击此处可从《天津医药》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号