伊维菌素光降解杂质的分离与鉴定

摘要：目的 分离和鉴定伊维菌素光降解杂质，为其储存条件选择提供依据。方法 伊维菌素精粉通过光降解试验得到高比例目标杂质，然后采用色谱纯化技术分离制备目标杂质化合物，再通过波谱分析鉴定杂质结构。结果 伊维菌素精粉在(4500±500)Lx条件下放置120h，目标降解杂质占比大于5.0%。通过单体化合物的制备和波谱分析，确定目标杂质为8a-hydroperoxyivermectin B1a。结论 建立了伊维菌素光降解杂质的制备方法，并通过杂质纯化和波谱分析，确定了杂质结构，为伊维菌素储存条件的选择提供了参考。

Isolation and identification of photodegradation impurities in ivermectin#br#

Abstract Objective To isolate and identify the photodegradable impurities of ivermectin, and provide reference for the selection of storage conditions. Methods High proportion of target impurities was obtained by photodegradation test of ivermectin refined powder. The target impurity was isolated by chromatography purification technology, and the structure of the impurity was identified by spectral analysis. Results More than 5.0% of the target degradation impurities was obtaied in degraded sample that was placed at (4500±500)Lx for 120 hours. The target impurity compound was prepared and identified，and it was named as 8a-hydroperoxyivermectin B1a.Conclusion The method of preparing ivermectin photodegradation impurity was established. The structure of the target impurity was identified by purification and spectral analysis, which provided a reference for the selection of storage conditions of ivermectin.