北京市鼠形动物感染巴尔通体的调查

目的 调查北京市鼠形动物巴尔通体感染状况和遗传特征。方法 采用夹夜法在北京市16个区捕获鼠形动物,使用PCR方法检测巴尔通体gltA基因,测序后进行系统发育树分析。结果 共采集7属9种鼠形动物样本448份,检测到巴尔通体阳性样本46份,总阳性率为10.3%,且阳性率在鼠种间、地区间和生境间的差异均具有统计学意义(X²_鼠种=114.980,X²_地区=22.133,X²_生境=96.466,P均<0.001)。系统发育树分析显示,40份样本序列位于进化树上的8个不同分支,提示北京市鼠形动物巴尔通体感染存在遗传多样性。结论 巴尔通体在北京市鼠形动物中广泛存在,并具有遗传多样性,本地人群存在一定的感染风险。

Investigation of Bartonella infection in murine-like animals in Beijing,China

This study aimed to investigate the infection and genetic characteristics of Bartonella in murine-like animals in Beijing. The murine-like animals were collected using the trap-at-night method in the 16 districts of Beijing and were tested for the Bartonella gltA gene by PCR. Phylogenetic tree analysis was performed on the basis of the gltA gene sequence. A total of 448 samples from 9 species of murine-like animals from 7 genera were collected, and 46 samples were positive for Bartonella sp., with a total positivity rate of 10.3%. The differences in positivity rates across species, regions and habitats were statistically significant (χspecies2=114.980, P<0.001; χregions2=22.133, P<0.001; χhabitats2=96.466, P<0.001). Phylogenetic tree analysis showed that the sequences of 40 positive samples clustered into 8 different branches on the phylogenetic tree. This result indicated the genetic diversity in Bartonella infected by murine-like animals in Beijing. In conclusion, Bartonella species with genetic diversity were widespread among murine-like animals in Beijing. There is a risk of local populations being infected by Bartonella.