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| 布鲁氏菌病患者尿液差异蛋白质组学分析 | |
| 引用本文： | 杨文涛,张建中,吴翠萍,王磊,赵利,倪秀莹,蓝峰,姜海,肖迪. 布鲁氏菌病患者尿液差异蛋白质组学分析[J]. 中国人兽共患病杂志, 2021, 37(7): 593-602. DOI: 10.3969/j.issn.1002-2694.2021.00.098 |
| 作者姓名： | 杨文涛 张建中 吴翠萍 王磊 赵利 倪秀莹 蓝峰 姜海 肖迪 |
| 作者单位： | 1. 包头医学院,包头 014040;2. 中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,感染性疾病诊治协同创新中心,北京 102206;3. 山东省潍坊市益都中心医院,益都 261200 |
| 基金项目： | “艾滋病和病毒性肝炎等重大传染病防治”科技重大专项(No.2018ZX10712001006012, No.2018ZX10733402003002) |
| 摘 要： | 目的 通过对布鲁氏菌病(简称布病)患者尿液蛋白进行通量化鉴定,发现与诊断布病相关的候选标示蛋白,并挖掘其参与的信号通路和生物标志物应用信息.方法 收集布病患者及非布病患者尿液样本各10例,采用基于质谱的非标定量蛋白质组学技术,发现与布病相关的差异表达蛋白,通过高通量生物信息学软件深入挖掘,确定差异表达蛋白参与的疾病相关... |
| 关 键 词： | 布鲁氏菌病 尿液 差异蛋白质组学 生物标志物 诊断标靶 |
| 收稿时间： | 2020-12-15 |
Differential proteomic analysis of urine in patients with brucellosis |
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| YANG Wen-tao,ZHANG Jian-zhong,WU Cui-ping,WANG Lei,ZHAO Li,NI Xiu-ying,LAN Feng,JIANG Hai,XIAO Di. Differential proteomic analysis of urine in patients with brucellosis[J]. Chinese Journal of Zoonoses, 2021, 37(7): 593-602. DOI: 10.3969/j.issn.1002-2694.2021.00.098 | |
| Authors: | YANG Wen-tao ZHANG Jian-zhong WU Cui-ping WANG Lei ZHAO Li NI Xiu-ying LAN Feng JIANG Hai XIAO Di |
| Affiliation: | 1. Baotou Medical college, Baotou 014040, China;2. State Key Laboratory of Communicable Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China;3. Yidu Central Hospital, Weifang City, Shandong Province,Yidu 261200, China |
| Abstract: | To identify potential urine marker proteins for the diagnosis of brucellosis, the urine samples of 10 patients with brucellosis and 10 people without brucellosis were identified by UPLC-MS/MS. On the basis of label-free quantitative proteomics, the raw data were searched in Proteome Discoverer software, and IPA software was used to analyze canonical pathways and biomarker applications of differentially expressed proteins. Finally, 1 817 proteins were identified, among which 185 differentially expressed proteins (13 up-regulated and 172 down-regulated) were screened according to |fold change (log2)| >1.5 and BH correction P<0.05. Seventeen previously unreported proteins were detected in human urine and showed differential expression in brucellosis. IGF2 was not found in the urine samples of patients with brucellosis. IGF2, CD300LF, ORM1, ORM2, LRG1, SH3BGRL3, CRIM1 and GLUL are potential urine marker proteins for diagnosing brucellosis and predicting its complications. This study not only provides new biomarkers for the rapid diagnosis of brucellosis and the discovery of drug targets, but also provides molecular clues for revealing the pathogenesis of this disease. |
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