2019年广州市甲型H1N1流感病毒全基因组序列的遗传特征分析

目的 对甲型H1N1流感病毒进行基因组全序列遗传特征分析,为流感的科学防控提供新数据.方法 选取7株2019年广州市甲型H1N1流感病毒进行全基因组序列测定,分析其遗传特征.结果 7株病毒的核苷酸同源性最高为PB2基因(98.8％～99.9％),最低为NA基因(97.3％～99.2％).总体上,不同月份的H1N1流感分...

Genetic characteristics of influenza A(H1N1)pdm09 viruses in Guangzhou in 2019

In this study, the genetic characteristics of novel influenza A (H1N1)pdm09 viruses were analyzed, to provide research data for the scientific prevention and control of influenza. Seven strains of novel influenza A (H1N1)pdm09 viruses in Guangzhou in 2019 were selected for sequencing. The genetic characteristics were analyzed in bioinformatics software. Among the eight segments of the seven viruses, the nucleotide sequence similarity of PB2 genes was the highest (98.8%~99.9%) and that of NA genes was the lowest (97.3%~99.2%). In general, the distribution of H1N1 influenza isolates in different months was time-dependent. The isolates after June in 2019 had the closest genetic relationship to the vaccine recommended strain (A/Guangdong Maonan/1536/2019 H1N1), and they were in the same evolutionary branch. Except for PA and M1 proteins, all proteins of the viruses from this branch had various amino acid mutations. The strains isolated from the influenza-like cases in monitoring hospitals belonged to the same evolutionary branch as those from the cases of community influenza outbreaks. The phylogenetic tree of each gene showed that there was no recombination among different genes from different sources. In conclusion, the strains of novel influenza A (H1N1) from monitoring cases in Guangzhou showed homology with the strains from the cases of influenza outbreaks in the community. The whole genome sequences matched the recommended vaccine strain well. No recombination was found.