变应原体外检测技术研究进展

变应原的准确检测对过敏性疾病的预防和诊疗至关重要.体外检测是当前变应原检测的主要研究方向.本文主要对检测血清sIgE的放射变应原吸附试验、酶联免疫吸附试验、荧光酶免疫分析、纳米磁微粒化学发光法、免疫印迹法、液相芯片法,以及直接检测变应原的生物共振法进行了概述,并对各种体外检测技术的优缺点进行了比较,以期为临床上选择合适...

Advances in detection of allergens in vitro technique

Accurate detection of allergens is vital for the prevention,diagnosis and treatment of allergic conditions. Currently, in vitrotechnique represents the leading research interest in allergen detection. This paper summarizes the available detection techniques targeting at serum sIgE measurement,including radioallergosorbent test,enzyme-linked immunosorbent assay,fluoroenzyme immunoassays,magnetic nano-particle chemiluminescence immunoassay,immunoblotting assay,liquidphase chip method as well as bioresonance method for direct detection of the allergens. In addition,the advantages and disadvantages are also compared pertinent to various in vitro detection techniques,with an attempt to provide reference for clinical option in selection of appropriate in vitrotechnique in detecting allergen in specific subjects.