circ_0000989通过调控miR-183-5p/LRP6轴促进视网膜母细胞瘤细胞增殖、迁移和侵袭

目的:探究环状RNA circ_0000989在视网膜母细胞瘤(retinoblastoma，RB)中的表达情况，及其对RB细胞功能的影响和调控机制。方法:采用实时荧光定量聚合酶链反应（qRT-PCR）检测RB组织和细胞中circ_0000989的表达水平。采用CCK-8和Transwell检测circ_0000989过表达质粒和miR-183-5p mimics对细胞增殖、迁移和侵袭的影响。采用Western blot检测circ_0000989和miR-183-5p对LRP6表达水平的影响。采用双荧光素酶报告基因分析circ_0000989对miR-183-5p的调控。结果:RB组织和细胞中circ_0000989表达显著上调。circ_0000989可以显著促进RB细胞的增殖、迁移和侵袭；而miR-183-5p可减弱此作用。双荧光素酶报告基因和Western blot显示circ_0000989通过吸附miR-183-5p促进LRP6的表达。结论:circ_0000989通过调控miR-183-5p/LRP6轴促进RB细胞增殖、迁移和侵袭，为临床RB患者的诊断和治疗提供了潜在的分子靶点。

circ_0000989 promotes the proliferation,migration and invasion of retinoblastoma cells by regulating miR-183-5p/LRP6

Objective:To investigate the expression of circ_0000989 in retinoblastoma (RB) and its effect and regulation mechanism on RB cell function.Methods:qRT-PCR was used to detect the expression of circ_0000989 in retinoblastoma tissues and cells.CCK-8 and Transwell were used to investigate the effects of circ_0000989 and miR-183-5p mimics on the proliferation，migration and invasion of retinoblastoma cells.Western blot was used to detect the effect of circ_0000989 and miR-183-5p on LRP6 expression.Double luciferase reporter gene was used to determine the circ_0000989's regulation of miR-183-5p.Results:The expression of circ_0000989 in RB tissues or cells was significantly up-regulated.circ_0000989 promote the proliferation,migration and invasion of RB cells,while miR-183-5p reduce this effect.Double luciferase reporter gene and Western blot showed that circ_0000989 promoted LRP6 expression by adsorbing miR-183-5p.Conclusion:circ_0000989 promotes the proliferation,migration and invasion of retinoblastoma cells by regulating the miR-183-5p/LRP6 axis,which provides a potential molecular target for clinical diagnosis and treatment of retinoblastoma.