河北省19F型和19A型侵袭性肺炎链球菌耐药性分析及多位点序列分型分析

目的 了解河北省侵袭性肺炎链球菌(Streptococcus pneumoniae,Spn)19F型和19A型的耐药特征及多位点序列分型(multilocus sequence type,MLST)分布.方法 19F 型和 19A 型采用多重聚合酶链式反应(multiplex polymerase chain reac...

Antibiotic resistance and multi-locus sequence typing of Streptococcuspneumoniae invasive serotypes 19F and 19A isolates in Hebei Province

Objective To investigate the antibiotic resistance characteristics of Streptococcus pneumoniae invasive serotypes 19F and 19A isolates and the distribution of multi-locus sequence type (MLST) in Hebei Province. Methods Multiplex polymerase chain reaction (mPCR) assay was used to determine the serotypes 19F and 19A. Microdilution broth method was employed to test the antibiotic resistance to 11 drugs. The minimum inhibitory concentration (MIC) was determined, and the antibiotic resistance was analyzed. MLST was performed to determine the sequence types (STs) of serotypes 19F and 19A. Results Fifteen strains of Streptococcus pneumoniae invasive serotype 19F were completely sensitive to linezolid, vancomycin and levofloxacin hydrochloride. Nine strains of Streptococcus pneumoniae invasive serotype 19A were completely susceptible to chloromycetin, linezolid, vancomycin, cefotaxime and levofloxacin hydrochloride. Four STs were detected in fifteen strains of Streptococcus pneumoniae invasive serotype 19F, three of which belonged to the new STs, and the dominant ST was ST-271 (9 strains, 60.00%). Two STs were detected in nine strains of Streptococcus pneumoniae invasive serotype 19A, and the dominant ST was ST-320 (8 strains, 88.89%). Conclusion The antibiotic resistance of Streptococcus pneumoniae invasive serotypes 19F and 19A isolates in Hebei Province is serious, and there exists a multiple drug resistance pattern. The genotypes of Streptococcuspneumoniae invasive serotype 19F are variable and complex, while the genotypes of Streptococcus pneumoniae invasive serotype 19A are compact.