血浆外泌体miR-423-5p在前列腺癌骨转移患者中的差异表达分析

目的:研究血浆外泌体来源microRNA(miRNA),作为前列腺癌骨转移的潜在标志物.方法:选取3例初诊为前列腺癌骨转移患者和3例局限性前列腺癌患者,收集患者外周静脉血10 mL,使用超速离心法及透射电子显微镜对血浆外泌体提取和鉴定.应用高通量测序分析血浆外泌体miRNA的差异表达谱.通过基因数据库GEO和已发表文章...

Analysis of differential expression of plasma exosome miR-423-5p in patients with prostate cancer bone metastasis

Objective:To study microRNA (miRNA) derived from plasma exosomes as a potential marker for bone metastasis of prostate cancer.Methods:We select three initially diagnosed patients with bone metastases from prostate cancer and three patients with primary prostate cancer,then collected 10 mL of peripheral venous blood from the patients and extract and identify exosomes by ultracentrifugation and transmission electron microscopy.High-throughput sequencing was used to analyze the differential expression profile of plasma exosome miRNA.We screened differential miRNAs through the genetic database GEO and published articles and selected differentially expressed miRNAs in the sequencing results.The plasma exosomes from 7 patients with primary prostate cancer and 10 patients with prostate cancer bone metastasis were verified by qRT-PCR,and also verified by combining with the GEO database samples.Results:High-throughput sequencing results show that an average of 740 miRNAs were detected from the patient's peripheral plasma exosomes,and differential miRNAs were calculated by R software to obtain 61 differential miRNAs.Compared with the control group,hsa-miR-885-5p,hsa-miR-1255a,hsa-miR-122-5p,hsa-miR-141-3p,hsa-miR-423-5p,hsa-miR-1224-5p and so on were differentially expressed in the bone metastasis group.The miRNA is selected by target gene function and database expression for PCR verification.It is found that the difference in hsa-miR-423-5p expression is statistically significant (P=0.033).According to the statistics of 60 patients with primary prostate cancer and 40 patients with bone metastasis through the database,hsa-miR-423-5p is significantly down-regulated in prostate cancer bone metastasis (P=0.001<0.05).Conclusion:The plasma exosome-derived miR-423-5p is significantly down-regulated in prostate cancer bone metastasis,and is expected to become a new marker in patients with prostate cancer bone metastasis.