miR-132通过靶向KLF7抑制鼻咽癌细胞增殖和迁移

目的探讨miR-132靶向负调节Kruppel样因子7(KLF7)表达对鼻咽癌(NPC)细胞增殖和迁移的影响。荧光定量PCR(qPCR)检测NPC组织和细胞及正常鼻咽组织和细胞中miR-132表达,Western blot检测KLF7蛋白表CNE-2Z细胞增殖、迁移和侵袭能力,裸鼠成瘤实验、免疫组化法检测CNE-2Z细胞体内生长、瘤内血管情况。结果鼻咽癌组织较正常鼻咽组织miR-132表达水平降低,KLF7蛋白表达增加;鼻咽癌CNE-2Z细胞较正常鼻咽上皮细胞NP69 miR-132表达水平降低,KLF7蛋白表达增加(P<0.05)。TargetScan预测及双荧光素酶检测显示,KLF7是miR-力,迁移和侵袭数量,肿瘤体积及微血管密度(MVD)显著降低(P<0.05),KLF7-OE组结果均相反(P<0.05)。相比miR-132 mimics组,miR-132 mimics+KLF7-OE组KLF7蛋白表达水平,36、48、60 h细胞增殖活力,迁移和侵袭数量、肿瘤体积及MVD显著升高(P<0.05)。结论 miR-132可通过负调控KLF7表达,影响NPC...

miR-132 inhibits proliferation and migration of nasopharyngeal carcinoma cells by targeting KLF7

Objective To investigate the effects of miR-132 on the proliferation and migration of nasopharyngealcarcinoma (NPC) cells by negatively targeting the expression of Kruppel-like factor 7 (KLF7). Methods NPC CNE-2Zcells were divided into blank group, NC group, miR-132 mimics group, KLF7-OE group and miR-132 mimics + KLF7-OEgroup. The expressions of miR-132 in NPC tissues and cells and normal nasopharyngeal tissues and cells were detected byfluorescence quantitative PCR (qPCR). The expression level of KLF7 protein was detected by Western blot assay. Thetargeting relationship between KLF7 and miR-132 was predicted and verified by TargetScan website and dual luciferasedetection. CCK-8 test and Transwell test were used to detect the proliferation, migration and invasion of CNE-2Z cells. Thetumor formation test in nude mice and immunohistochemistry were used to detect the growth of CNE-2Z cells in vivo and theblood vessels in tumor. Results The expression level of miR-132 was decreased in nasopharyngeal carcinoma tissue thanthat in normal nasopharyngeal tissue, and the expression of KLF7 protein was increased. The expression level of miR-132was decreased in nasopharyngeal carcinoma CNE-2Z cells than that in normal nasopharyngeal epithelial cells NP69, and theexpression of KLF7 protein was increased (P＜0.05). TargetScan prediction and dual luciferase detection showed that KLF7was the target gene of miR-132. Compared with those in the blank group and NC group, the expression of KLF7 protein, cellproliferation activity at 36, 48 and 60 h, numbers of migration and invasion, tumor volume and microvessel density (MVD) inCNE-2Z cells were significantly decreased in miR-132 mimics group (P＜0.05), while the results were oppositive in KLF7-OE group (P＜0.05). Compared with those in miR-132 mimics group, the expression level of KLF7 protein, cell proliferationactivity at 36, 48 and 60 h, numbers of migration and invasion, tumor volume and MVD were significantly increased in miR-132 mimics + KLF7-OE group (P＜0.05). Conclusion miR-132 can affect the proliferation, migration and invasion ofNPC cells by negatively regulating the expression of KLF7.