花生四烯酸对小鼠日本血吸虫病治疗效果观察

目的 观察花生四烯酸对小鼠体内日本血吸虫的杀虫效果。方法 小鼠感染日本血吸虫尾蚴后,被随机分为给药组、溶剂组和未处理组。给药组小鼠在不同给药时间点,以300 mg/kg的剂量连续2 d或连续7 d灌服花生四烯酸溶液,溶剂组小鼠在相应时间点灌服玉米油,各组小鼠于感染后42 d解剖,收集小鼠体内成虫、肝内虫卵并计数,计算减虫率和减卵率。结果 300 mg/kg花生四烯酸对小鼠体内日本血吸虫成虫(28~35 d)有杀虫效果,减虫率超过40%,肝内减卵率超过70%,与溶剂组的差异均有统计学意义(P<0.05)。300 mg/kg花生四烯酸对小鼠体内童虫期(1~28 d)日本血吸虫没有明显的杀虫效果。结论 花生四烯酸对日本血吸虫成虫有杀虫作用,具有被用于日本血吸虫病药物治疗的潜力。

Therapeutic effect of arachidonic acid (ARA) on Schistosomiasis japonicum in mice

To reveal ARA’s effects against Schistosoma japonicum in BALB/c and C57BL/6j mouse hosts, we randomly divided mice infected with S. japonicum cercariae into three groups: an administration group, solvent group and untreated group. Mice in the administration group were given ARA solution at a dose of 300 mg/kg for 2 consecutive days or 7 consecutive days at different time points, whereas mice in the solvent group were given only corn oil. Mice in each group were dissected on the 42nd day after infection, and adult worms and liver eggs were collected and counted to calculate the worm and egg reduction rates. ARA effectively suppressed adult Schistosoma japonicum (28-35 days) in mice at the dose of 300 mg/kg, with a worm reduction rate above 40% and a liver egg reduction rate of more than 70%; the differences between the administration and the solvent groups were statistically significant. However, ARA at the same concentration had no obvious effects against S. japonicum during the schistosomulum stage (1-28 days) in mice. The results revealed that S. japonicum adult worms are sensitive to ARA; therefore, ARA has the potential to be used in the chemical treatment of S. japonicum in the future.