miR-1246在肝癌血清中的表达及对肝癌HepG2细胞生物学功能的影响

  目的  探讨miR-1246在肝癌患者血清中的表达及其对HepG2细胞生物学功能的影响。  方法  应用荧光定量PCR检测miR-1246在正常对照者和肝癌患者血清中的表达；将miR-1246 mimics和NC mimics分别转染至HepG2细胞，采用Transwell小室法、划痕实验和细胞克隆形成实验检测miR-1246对HepG2细胞生物学功能的影响，荧光定量PCR检测NFE2L3、Vimentin、GAS1基因的表达改变。  结果  miR-1246在正常对照者和肝癌患者血清中的表达量分别为1.079(0.740，1.534)、0.132(0.068，0.324)，差异有统计学意义(P < 0.001)；miR-1246 mimics转染HepG2细胞后，HepG2细胞穿过基质胶的细胞数为128.000±9.900, 较NC mimics组(251.500±24.749)明显减少(P=0.023)，划痕48 h后HepG2细胞相对迁移率为(24.833±3.351)%，较NC mimics组(31.859±7.194)%明显减慢(P=0.046)，Vimentin、GAS1、NFE2L3基因相对表达量分别为0.318±0.213、0.479±0.157、0.755±0.044，较NC mimics组(1.000±0.000)表达均明显下调(P=0.046、0.043、0.030)。  结论  肝癌患者血清中miR-1246表达下调；miR-1246可能通过下调Vimentin、GAS1、NFE2L3基因抑制HepG2细胞的侵袭、迁移能力。 

Expression of microRNA-1246 in the serum of hepatocellular carcinoma and its effect on biological function of HepG2 cells

  Objective  To investigate the expression of microRNA-1246 (miR-1246) in hepatocellular carcinoma (HCC) serum and its effect on the biological function of HepG2 cells.  Methods  Serum miR-1246 was determined by qRT-PCR in HCC patients and healthy controls. MiR-1246 mimics and NC mimics were transfected into HepG2 cells separately. The effect of miR-1246 on the biological function of HepG2 cells was examined by transwell chamber assay, scratch test and cell clone formation test. The expression of NFE2L3, Vimentin and GAS1 were detected by qRT-PCR.  Results  The expression levels of miR-1246 in the serum of healthy controls and HCC patients were 1.079 (0.740, 1.534) and 0.132 (0.068, 0.324), respectively, and the difference was statistically significant (P < 0.001). After transfection of miR-1246 mimics into HepG2 cells, the number of HepG2 cells passing through matrigel was (128.000±9.900), which was significantly lower than that of the NC mimic group (251.500±24.749, P=0.023). Forty-eight hours after scratch, the relative mobility of HepG2 cells was (24.833±3.351) %, which was significantly lower than that in the NC mimic group (31.859±7.194) % (P=0.046). The relative expression levels of Vimentin, GAS1 and NFE2L3 were (0.318±0.213), (0.479±0.157) and (0.755±0.044), respectively, which were significantly lower than those in the NC mimic group (P=0.046, P=0.043 and P=0.030, respectively).  Conclusion  The expression levels of miR-1246 were decreased in HCC serum. MiR-1246 could inhibit the invasion and migration of HepG2 cells by down-regulating the expression of Vimentin, GAS1 and NFE2L3.