CXCL10、CXCR3在成年人结节性胃炎患者胃黏膜中的表达及意义*

目的:探讨趋化因子CXCL10及其受体CXCR3在成年人结节性胃炎患者胃黏膜中的表达及意义。方法:选取2020年4月-2021年11月就诊于内蒙古科技大学包头医学院第二附属医院消化病研究所胃镜室的患者,根据内镜下表现、病理学检查及幽门螺旋杆菌(Hp)呼气试验筛选结节性胃炎患者及对照组患者共64例,将患者分为结节性胃炎组(NG组)33例、Hp阳性的慢性非萎缩胃炎组14例、Hp阴性的慢性非萎缩胃炎组17例,其中结节性胃炎组均有Hp感染,收集胃黏膜标本,采用免疫组化、Western-blot等技术检测各组患者胃黏膜CXCL10、CXCR3蛋白阳性染色、蛋白表达量。结果:免疫组化结果显示,在胃黏膜中,NG组、Hp阳性慢性非萎缩胃炎组、Hp阴性慢性非萎缩胃炎组间CXCL10、CXCR3蛋白均有阳性表达,各组间弱阳性表达较多,其中Hp阳性慢性非萎缩胃炎组胃黏膜内CXCL10、CXCR3染色阳性率分别为87.5 %(7/8)、75.0 %(6/8),但各组间差异均无统计学意义(P＞0.05)。Western-blot结果显示:与NG组和Hp阴性慢性非萎缩胃炎组相比,Hp阳性慢性非萎缩胃炎组CXCL10、CXCR3蛋白表达更为显著,差异有统计学意义(P＜0.05)。结论:成年人结节性胃炎与Hp感染密切相关,CXCL10、CXCR3在Hp感染患者胃黏膜中表达增加,而在结节性胃炎中表达降低可能代表免疫反应的普遍抑制。

Expression and significance of CXCL10 and CXCR3 in gastric mucosa of adult patients with nodular gastritis

Objective: To investigate the expression and significance of chemokine CXCL10 and its receptor CXCR3 in gastric mucosa of adult patients with nodular gastritis. Methods: Patients who were admitted to the gastroscope room of the Institute of Digestive Diseases, Second Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology from April 2020 to November 2021 were selected according to endoscopic manifestations, pathological examinations and Helicobacter pylori (Hp) breath test A total of 64 patients with nodular gastritis and control group were screened, and the enrolled patients were divided into nodular gastritis group (NG group), 33 cases, Hp-positive chronic non-atrophic gastritis group, 14 cases, and Hp-negative chronic non-atrophic gastritis.There were 17 cases in the group, including the nodular gastritis group with Hp infection.The gastric mucosa samples were collected, and immunohistochemistry, Western-blot and other techniques were used to detect the positive staining and protein expression of CXCL10、CXCR3 protein in the gastric mucosa of the patients in each group. Results: Immunohistochemistry showed that in gastric mucosa, positive expressions of CXCL10 and CXCR3 were found among NG group, Hp-infected chronic non-atrophic gastritis group and non-Hp-infected chronic non-atrophic gastritis group, and there were more weak positive expressions among different groups.The positive staining rates of CXCL10 and CXCR3 in gastric mucosa of Hp-infected non-atrophic gastritis group were 87.5 %(7/8) and 75.0 %(6/8), but the differences between the groups were not statistically significant.Western-blot results showed that compared with the NG group and the non-Hp-infected chronic non-atrophic gastritis group, the protein expressions of CXCL10 and CXCR3 in the Hp-infected chronic non-atrophic gastritis group were more significant and the differences were statistically significant (P＜0.05). Conclusion: Nodular gastritis in adults is closely related to Hp infection.The expression of CXCL10、CXCR3 is increased in gastric mucosa of patients with Hp infection, and decreased expression in nodular gastritis may represent universal inhibition of immune response.