甘肃黄鼠疫源地鼠疫宿主动物致病性耶尔森菌调查分析

目的 了解甘肃阿拉善黄鼠疫源地内鼠疫宿主动物致病性耶尔森菌感染状况,为探索该疫源地鼠疫流行状态提供依据。方法 2016-2018年现场采集阿拉善黄鼠鼠疫疫源地会宁县、平川区鼠疫宿主动物阿拉善黄鼠及花鼠、大仓鼠、沙土鼠、子午沙鼠、中华鼢鼠、小灰鼠、达乌尔鼠兔、小家鼠、灰仓鼠的回盲部及内容物、舌根部、血清标本,分别进行耶尔森菌分离及鼠疫F1抗体的检测。结果 会宁县共采集阿拉善黄鼠及花鼠、大仓鼠、沙土鼠、子午沙鼠、中华鼢鼠舌根部标本274份,未检出耶尔森菌,回盲部及内容物标本275份,检出1株非致病性小肠结肠炎耶尔森菌,其毒力型别为(ail- ystA- ystB+ yadA- virF- rfbc-),检菌率为0.18%;平川区共采集阿拉善黄鼠及沙土鼠、子午沙鼠、中华鼢鼠、小灰鼠、达乌尔鼠兔、小家鼠、灰仓鼠舌根部标本213份,回盲部及内容物标本219份均未检出耶尔森菌。阿拉善黄鼠血清312份,鼠疫F1抗体结果均为阴性。结论 甘肃黄鼠疫源地鼠类肠道内可能存在耶尔森菌群,检出的小肠结肠炎耶尔森菌的地点与阿拉善黄鼠鼠疫菌的地点一致。

Investigation and analysis of pathogenic yersinia in plague host animals in the Spermonphilus alaschanicus plague foci of Gansu Province

This study aimed to understand the status of Yersinia pestis infection in host animals in the plague foci of Spermonphilus alaschanicus in Gansu and to provide a basis for exploring the epidemic status of plague in these foci. Samples of the ileocecal region and contents, pharyngeal swabs or tongue roots, and serum samples from plague host animals in Huining and Pingchuan during the 1960s and 1970s were collected on site from 2016 to 2018. A total of 274 specimens from the tongue base in six species of mice were collected in Huining County, and 275 specimens from the ileocecal region and contents were collected. One non-pathogenic strain of Yersinia enterotidis was detected, and its virulence type was ail- ystA- ystB+ yadA- virF- rfbc-, with a detection rate of 0.18%. A total of 213 tongue base specimens from eight species of rodents were collected in Pingchuan district. No yersinia was detected in 219 ileocecal and content specimens. A total of 312 serum samples of Alxa yellow squirrel serum samples were collected. The plague F1 antibody results were negative. Yersinia may exist in the intestines of the main host animals in the source areas of bubonic plague in Gansu, and the sites with detected Yersinia enterocolitis are consistent with those in which Yersinia pestis was detected in the 1960s and 1970s.