长链非编码RNA HOXA-AS3在结肠癌组织中的表达及与临床预后的关系

目的:观察长链非编码RNA HOXA-AS3(lncRNA HOXA-AS3)在结肠癌组织中的表达并探究其与临床预后的关系.方法:选取2012年01月至2014年02月本院外科行手术治疗的94例结肠癌患者癌变及癌旁结肠组织冻存标本,采用实时定量PCR(RT-qPCR)法检测癌组织和癌旁组织中lncRNA HOXA-AS...

Expression of long non-coding RNA HOXA-AS3 in colon cancer and its relationship with clinical prognosis

Objective:To observe the expression of long non-coding RNA HOXA-AS3(lncRNA HOXA-AS3) in colon cancer tissues and explore its relationship with clinical prognosis.Methods:From January 2012 to February 2014,cryopreserved specimens of cancerous and adjacent colonic tissues of 94 patients with colon cancer underwent surgical treatment were collected.The expression of lncRNA HOXA-AS3 in cancer tissues and adjacent tissues was detected by real-time quantitative PCR(RT-qPCR).One-way ANOVA was used to analyze its relationship with clinical pathological parameters of patients with colon cancer.Kaplan-Meier survival curve was used to analyze the relationship between the expression of lncRNA HOXA-AS3 and the 5-year survival rate of patients with colon cancer,and COX multivariate regression analysis was used to analyze the risk factors of adverse prognosis in patients with colon cancer.Results:Compared with the adjacent tissues,the expression of lncRNA HOXA-AS3 in colon cancer tissues increased significantly (P<0.05).The expression level of lncRNA HOXA-AS3 in colon cancer tissues was not related to age,gender and pathological type of patients (P＞0.05),but was related to TNM stage,tumor diameter,differentiation degree and lymph node metastasis (P<0.05).The 5-year survival rate of colon cancer patients with high expression of lncRNA HOXA-AS3 (60.98%) was significantly lower than that of low expression group (94.34%) (χ2=16.41,P<0.05).COX regression analysis showed that high expression of lncRNA HOXA-AS3,TNM stage and lymph node metastasis were independent risk factors for prognosis of colon cancer patients (P<0.05).Conclusion:lncRNA HOXA-AS3 is highly expressed in colon cancer tissues,and is related to TNM stage,tumor diameter,differentiation,lymph node metastasis and 5-year survival rate.The expression of lncRNA HOXA-AS3 may be related to the occurrence and development of colon cancer.It has potential to become a new biological indicator and potential therapeutic target for prognosis evaluation of colon cancer.