miR-34a-3p、DDX27在结直肠癌组织中的表达水平及临床意义

目的:检测结直肠癌患者组织中微小RNA-34a-3p（miR-34a-3p）、DEAD盒多肽27（DDX27）的表达水平，并探讨二者表达水平与结直肠癌预后的相关性。方法:选取2016年01月至2017年04月于本院住院的102例结直肠癌癌组织作为结直肠癌组，选取其癌旁正常组织作为癌旁对照组。采用实时荧光定量PCR（qRT-PCR）法检测组织miR-34a-3p、DDX27 mRNA表达水平；分析结直肠癌患者组织中miR-34a-3p、DDX27 mRNA表达水平与患者临床病理特征的关系；Kaplan-Meier法分析结直肠癌组织中miR-34a-3p、DDX27表达水平与患者生存率的相关性；多因素Cox回归分析影响结直肠癌预后的因素。结果:Targetscan网站在线预测结果显示miR-34a-3p与DDX27的3' UTR之间存在碱基互补关系。双荧光素酶实验结果显示，与miR-NC相比，miR-34a-3p-mimics与野生型3' UTR区（WT-DDX27）共转出现了荧光减弱，而与突变3' UTR区（MUT-DDX27）共转后荧光强度未出现明显变化；Ualcan数据库中，结肠癌组织中DDX27水平高于正常结肠组织（P＜0.05）；结直肠癌组DDX27 mRNA水平高于癌旁对照组，miR-34a-3p水平低于癌旁对照组（P＜0.05），且DDX27表达趋势与Ualcan数据库一致；miR-34a-3p、DDX27表达与肿瘤大小、组织分化程度、淋巴结转移、TNM分期有关（P＜0.05）；miR-34a-3p低表达组三年内存活率（64.00%）低于高表达组（94.23%），DDX27高表达组三年内存活率（66.67%）低于低表达组（92.16%），差异均有统计学意义（P＜0.05）；DDX27、组织分化程度、TNM分期是影响结直肠癌不良预后的独立危险因素（P＜0.05），miR-34a-3p是影响结直肠癌不良预后的保护因素（P＜0.05）。结论:结直肠癌组织中miR-34a-3p、DDX27表达水平与组织分化程度、TNM分期等临床病理特征密切相关，且均是影响结直肠癌预后的因素，可对结直肠癌的病情评价及治疗提供一定理论依据。

Expressions level and clinical significance of miR-34a-3p and DDX27 in colorectal cancer tissues

Objective:To detect the expression level of microRNA-34a-3p(miR-34a-3p) and DEAD box polypeptide 27(DDX27) in colorectal cancer,and to explore the relationship between the expression levels and colorectal cancer.Methods:From January 2016 to April 2017,102 cases of colorectal cancer patients in our hospital were selected as colorectal cancer group,and their normal paracancerous tissues were selected as the control group.The levels of miR-34a-3p and DDX27 mRNA were detected by real-time fluorescent quantitative PCR(qRT-PCR).The relationships between miR-34a-3p,DDX27 mRNA levels and clinicopathological features in patients with colorectal cancer were analyzed.Kaplan-Meier method was used to analyze the relationships between the expressions of miR-34a-3p,DDX27 and the survival rate of colorectal cancer patients.Multivariate Cox regression was used to analysie the prognostic factors of colorectal cancer.Results:The online prediction results of Targetscan website showed that there was a base complementary relationship between miR-34a-3p and the 3' UTR of DDX27.The results of the dual luciferase experiment showed that,compared with miR-NC,the fluorescence of miR-34a-3p-mimics co-transformed with the wild-type 3' UTR region(WT-DDX27) was reduced,and compared with the mutant 3' UTR region(MUT-DDX27),the luorescence intensity did not change significantly after co-transformation.In the Ualcan database,the level of DDX27 in colon cancer tissues was higher than that in normal colon tissues(P＜0.05).The level of DDX27 mRNA in colorectal cancer group was higher than that in the paracancerous control group,the level of miR-34a-3p was lower than that of the paracancerous control group(P＜0.05),and the expression trend of DDX27 was consistent with that of ualcan database.The expressions of miR-34a-3p and DDX27 were related to tumor size,tissue differentiation,lymph node metastasis and TNM stage(P＜0.05).The three-year survival rate of miR-34a-3p low expression group(64.00%) was lower than that of high expression group(94.23%),and the three-year DDX27 of survival rate high expression group(66.67%) was lower than that of low expression group(92.16%),the differences were statistically significant(P＜0.05).DDX27,tissue differentiation and TNM stage were independent risk factors for poor prognosis of colorectal cancer(P＜0.05),while miR-34a-3p was a protective factor for poor prognosis of colorectal cancer(P＜0.05).Conclusion:The levels of miR-34a-3p and DDX27 are closely related to the degree of tissue differentiation,TNM stageand other clinicopathological features.All of them are factors influencing the prognosis of colorectal cancer,which can provide a theoretical basis for the evaluation and treatment of colorectal cancer.