猪链球菌2型强弱毒株对BALB/c小鼠肠道菌群影响的差异

目的 旨在探究猪链球菌2型强弱毒株对BALB/c小鼠肠道菌群的影响差异.方法 本研究随机将3周龄的雌性BALB/c小鼠分为3组,即空白对照组、猪链球菌2型强毒株SS2-1感染组和弱毒株HA0609感染组.采用Illumina Miseq测序技术,测定了小鼠结肠粪便样品中微生物16S rRNA V3-4区序列,并对其微生...

Different effects of the virulent and avirulent strains of Streptococcus suis type 2 on the intestinal microbiota in BALB/c mice

The objective of this study was to investigate the effects of two Streptococcus suis type 2 strains, the virulent strain SS2-1 and the avirulent strain HA0609, on the intestinal microbiota in BALB/c mice. Three week old female mice were randomly divided into three groups: a control group (Mock), an SS2-1 strain infected group and an HA0609 strain infected group. The 16S rRNA v3-4 regions of the intestinal microbiota were sequenced on the Illumina Miseq platform, and the community structure and diversity of intestinal microbiota were analyzed according to the sequence data. The sequencing coverage of all samples in each group exceeded 99%, thus indicating that the experimental data were reliable. Marked differences were observed in the abundance and communities of intestinal microbiota among the three groups. Both the number of OTU and the alpha diversity of the intestinal microbiota were higher in the SS2-1 group than the control and HA0609 groups. The HA0609 group showed greater similarity to the control group. An increased proportion of pathogenic bacteria or potential pathogenic bacteria and a decreased proportion of probiotics were detected in the SS2-1 group, thus demonstrating the imbalance in the intestinal microbiota. According to the results of LEfSe analysis, Alphaproteobacteria may be used as a potential biomarker for diagnosis of Streptococcus suis type 2 infection. The results of this study not only provide insights into the abundance, diversity and composition of the intestinal microbiota of BALB/c mice infected with Streptococcus suis type 2 virulent or avirulent strains, but also identify the potential biomarker Alphaproteobacteria for diagnosis of Streptococcus suis type 2. This study provides experimental data for improving treatment and preventing Streptococcus suis type 2 infection by adjusting microorganisms.