miR-199a通过下调ITGA3抑制卵巢癌细胞的增殖、迁移与侵袭

目的:探讨miR-199a对卵巢癌细胞增殖、迁移与侵袭的作用及机制。方法:采用qRT-PCR和Western blot分别检测卵巢癌组织和细胞中miR-199a和整合素α3（integrin alpha 3，ITGA3）的表达。将miR-199a mimic转染入CAR3细胞后，双荧光素酶报告基因实验、Western blot检测miR-199a对ITGA3的调控作用；MTT和Transwell实验研究了miR-199a和ITGA3对CAR3细胞增殖、迁移与侵袭的作用。结果:卵巢癌组织和细胞中miR-199a表达降低（P＜0.01），ITGA3表达增加（P＜0.01）。双荧光素酶报告基因实验结果显示miR-199a与ITGA3 3'-UTR有结合作用。Western blot结果证实miR-199a可抑制ITGA3表达（P＜0.01）。miR-199a过表达抑制CAR3细胞增殖、迁移与侵袭（P＜0.01）；而过表达ITGA3能逆转miR-199a过表达的作用（P＜0.01）。结论:miR-199a抑制卵巢癌细胞增殖、迁移与侵袭的作用可能与其通过下调靶基因ITGA3表达相关。

miR-199a inhibits proliferation,migration and invasion of ovarian cancer cells by down-regulation of ITGA3

Objective:To investigate the effect and mechanism of miR-199a on the proliferation,migration and invasion of ovarian cancer cells.Methods:The expressions of miR-199a and integrin alpha 3 (ITGA3) in ovarian cancer tissues and cells were detected by qRT-PCR and Western blot,respectively.After miR-199a mimic was transferred into CAR3 cells,the regulatory effects of miR-199a on ITGA3 were detected by dual-luciferase reporter assay and Western blot.The effects of miR-199a and ITGA3 on the proliferation,migration and invasion were detected by MTT and Transwell assays.Results:The expression of miR-199a was decreased in ovarian cancer tissues and cells (P＜0.01),and the expression of ITGA3 was increased (P＜0.01).The result of dual-luciferase reporter assay showed that miR-199a binded to ITGA3 3'-UTR.The result of Western blot confirmed that miR-199a could inhibit the expression of ITGA3 (P＜0.01).Over-expression of miR-199a inhibited proliferation,migration and invasion of CAR3 cells (P＜0.01).However,over-expression of ITGA3 reversed the effect of over-expression of miR-199a (P＜0.01).Conclusion:The inhibitory effect of miR-199a on the proliferation,migration and invasion of ovarian cancer cells may be related to the down-regulation of the target gene ITGA3 expression.