杞菊地黄胶囊防治激光性视网膜损伤的作用机制:基于网络药理学探讨

目的 运用网络药理学探讨杞菊地黄胶囊防治激光性视网膜损伤的作用机制.方法 基于中药系统药理学数据库,获取杞菊地黄胶囊的活性成分及其潜在靶点,通过口服生物利用度≥30％和类药性≥0.18,筛选出有效成分.在Therapeutic Target Database数据库、Drugbank3.0数据库、DisGeNET 5.0...

Mechanism of Qiju Dihuang capsule in the prevention and treatment of laser-induced retinal injury:a discussion based on network pharmacology

Objective　To explore the mechanism of Qiju Dihuang capsule in preventing and treating laser-induced retinal injury by means of network pharmacology.Methods　The active components and potential targets of Qiju Dihuang capsule were obtained based on the systematic pharmacological database of traditional Chinese medicine, and the active components were screened by oral bioavailability ≥ 30% and drug-like ≥ 0.18. The potential targets of laser-induced retinal injury were searched in therapeutic Target Database, Drugbank3.0 database and DisGeNET5.0 database with “laser induced retinal injury” as the keyword, and the potential targets were introduced into Uniport database for gene standardization. After deleting duplicates and unable to standardize target genes, they were integrated and summarized as potential target genes of diseases. Wayne diagram was used to select the intersection of drug targets and disease targets as efficacy targets, and then these efficacy targets were imported into String database for analysis, and the core network map of protein-protein interaction was established. The target was enriched and analyzed by Kyoto Encyclopedia of Gene and Genome and Gene Ontology.Results　Totally 139 active components were obtained by screening oral bioavailability and drug-like parameters. The targets corresponding to active components were introduced into Uniprot online database for gene standardization, and 238 component targets were obtained. There were 911 targets related to laser-induced retinal injury, such as tumor necrosis factor, interleukin(IL)-6, TP53, IL-10 and so on. The results of gene ontology enrichment analysis showed that the targets of Qiju Dihuang capsule in the treatment of laser-induced retinal injury were mainly related to the pathway of cancer, AGE-RAGE signal pathway, IL-17 signal pathway, human T cell leukemia virus 1 infection, HIF-1 signal pathway, platinum resistance, leishmaniasis, NF-κB signal pathway and so on. The results of functional annotation enrichment analysis of Kyoto Encyclopedia of Gene and Genome showed that the related targets were mainly concentrated in AGE-RAGE signal pathway, IL-17 signal pathway, NF-κB signal pathway, HIF-1 signal pathway and so on.Conclusion　Qiju Dihuang capsule can inhibit oxidative stress, inflammation and immune response of retinal endothelial cells by regulating AGE-RAGE signal pathway, NF-κB signal pathway and HIF-1 signal pathway, thereby treating laser-induced retinal injury.