| 胶质母细胞瘤组织中沉默FAP-1表达促进细胞增殖和迁移 |
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| 引用本文： | 沙娅·玛哈提,刘 洋,肖双东,刘 文,王增亮.胶质母细胞瘤组织中沉默FAP-1表达促进细胞增殖和迁移[J].现代肿瘤医学,2021,0(11):1862-1866. |
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| 作者姓名： | 沙娅·玛哈提 刘 洋 肖双东 刘 文 王增亮 |
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| 作者单位： | 1.新疆医科大学第一附属医院肿瘤中心;2.神经外科中心，新疆 乌鲁木齐 830011 |
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| 基金项目： | National Natural Science Foundation of China(No.81801232）；国家自然科学基金青年科学基金项目（编号:81801232） |
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| 摘 要： | 目的:探讨FAP-1在胶质母细胞瘤(glioblastoma，GBM)组织中的表达及其对胶质瘤细胞增殖、迁移作用的影响。方法:通过Oncomine数据库探讨FAP-1在胶质瘤中的表达，并通过PCR测定胶质母细胞瘤组织及非胶质母细胞瘤组织中FAP-1的表达水平；通过不同浓度FAP-1 siRNA转染胶质瘤细胞系LN18、U87，降低FAP-1表达，通过吸光度实验观察FAP-1表达对胶质母细胞瘤细胞系增殖能力影响，划痕实验评估FAP-1对胶质瘤细胞系迁移能力影响。结果:MTT实验提示沉默FAP-1表达促进胶质母细胞瘤细胞增殖能力（P＜0.05）。划痕实验提示沉默FAP-1表达则促进胶质母细胞瘤细胞迁移能力。结论:FAP-1在胶质母细胞瘤中可能发挥着抑癌基因的功能。
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| 关 键 词： | 胶质瘤 FAP-1 增殖 迁移 |
Silencing expression of FAP-1 in glioblastoma tissues promote cell proliferation and migration |
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| Shaya·Mahati,LIU Yang,XIAO Shuangdong,LIU Wen,WANG Zengliang.Silencing expression of FAP-1 in glioblastoma tissues promote cell proliferation and migration[J].Journal of Modern Oncology,2021,0(11):1862-1866. |
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| Authors: | Shaya·Mahati LIU Yang XIAO Shuangdong LIU Wen WANG Zengliang |
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| Institution: | 1.Department of Tumor Center;2.Department of Neurosurgery Center,First Affiliated Hospital of Xinjiang Medical University,Xinjiang
Urumqi 830011,China. |
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| Abstract: | Objective:To investigate the expression and function of FAP-1 in glioblastoma and its effect on proliferation and metastasis of glioma cells.Methods:Oncomine database was used to examine the expression of FAP-1 in GBM and then PCR was performed to test the results,different concentration of FAP-1 siRNA targeted FAP-1 were applied to adjust the expression levels of FAP-1,scratch test and absorbance test demonstrate were performed to test the effect of FAP-1 on cell proliferation and migration.Results:MTT and scratch test suggested that knocked down of FAP-1 resulted in enhancement of cell proliferation and migration.Conclusion:FAP-1 may play a role of cancer suppressor gene in GBM. |
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| Keywords: | glioma FAP-1 proliferation migration |
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