藏药旺拉提取物对胆碱能损伤大鼠的神经保护作用

目的:研究藏药旺拉提取物(CE)对胆碱能损伤大鼠的神经保护作用。方法:采用基底前脑注射鹅蒿蕈氨酸的方法建立胆碱能损伤大鼠模型。实验动物分为假手术组,模型组,阳性药吡拉西坦(400 mg.kg-1),CE(0.31,1.25,5 mg.kg-1)组,连续灌胃给药28 d。跳台法检测大鼠学习记忆能力,尼氏染色法观察大鼠基底前脑尼氏小体的变化,免疫组化法观察海马CA1区神经生长因子的表达水平。结果:与假手术组大鼠相比,模型组大鼠跳下平台的潜伏期明显缩短,基底前脑尼氏小体数量减少,海马CA1区神经生长因子表达水平明显下降。与模型组大鼠相比,CE 5 mg.kg-1可改善记忆损伤大鼠在跳台试验中的表现(P<0.05),使基底前脑病理改变部分恢复正常,上调海马CA1区神经生长因子表达水平(P<0.05)。结论:CE具有神经保护作用,可改善胆碱能损伤大鼠学习记忆能力。

Neuroprotective Effect of Coeloglossum Extract on Cholinergic Impaired Rats

Objective: To study the protective effect of coeloglossum extract (CE), an exact isolated from a Tibetan traditional herb, against ibotenic acid (IBO)-induced cholinergic injury in rats. Method: Rats were randomly assigned into six groups: sham(NS), model(IBO), piracetam(IBO+400 mg·kg^-1),and CE(IBO+0.31,1.25,5 mg·kg^-1)groups. After 28 days, step-down test was employed to evaluate the learning and memory function, Nissl staining was adopted to detect the Nissl body changes in basal forebrain, and immunohistochemical staining was used to examine the expression of nerve growth factor (NGF) in hippocampus CA1. Result: Compared with the sham group, rats in model group exhibited a shorter latency to platform, and lower levels of nissl body in frontal cortexes and of NFG in hippocampus CA1.Compared with the model rats, CE(5 mg·kg^-1) prolonged significantly the latency of step-down test (P<0.05), partly recovered the pathological changes in basal forebrain, and increased the level of NGF in hippocampus CA1(P<0.05). Conclusion: These results suggested that CE can improve the cholinergic impairment and the disability of learning and memory in IBO rats.