Identification of CYP2C9*2 allele in HepG2 cell line |
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Authors: | Chen Jiezhong Raymond Kenneth |
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Institution: | (1) School of Pharmacy and Applied Science, Faculty of Science, Technology and Engineering, La Trobe University, Bendigo, Vic, 3550, Australia |
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Abstract: | Background Hepatoma is caused by many factors including alcohol, chemicals, viral infection, and chronic inflammation. Cytochrome P450
polymorphism plays an important role in its pathogenesis. CYP2D6, CYP2E1, and CYP1A1 have been identified to be related with
hepatic carcinogenesis and tumor size and stage. However, no studies have been performed on CYP2C9, a major CYP in the liver
and hepatoma.
Aim of the study To identify if there is polymorphism of CYP2C9 in a HepG2 cell line.
Methods A pair of primers was used to clone CYP2C9 exon 3 region and subsequently sequenced. The sequence was compared to normal CYP2C9
for identification of any mutation.
Results A point mutation was identified. It was located in the amino acid number 144 of CYP2C9 protein with the change of normal amino
acid arginine into cysteine, which is the same as identified in poor metabolism patients as homozygous CYP2C9*2.
Conclusions There is a mutation (CYP2C9*2/ CYP2C9*2) in a HepG2 cell line. Thus, polymorphism of CYP2C9 may also be involved in the carcinogenesis
of hepatoma as CYPs2D6 and 2E1. |
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Keywords: | polymorphism hepatoma CYP2C9 |
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