莱菔硫烷对食管癌细胞凋亡以及Caspase-3和Survivin表达的影响

目的研究莱菔硫烷（sulforaphane，SFN）对食管癌细胞凋亡的影响以及能否影响细胞凋亡蛋白酶（Caspase）-3和存活素（Survivin）的表达。方法培养食管癌细胞EC9706，用10—50μM的SFN处理后，流式细胞分析术检测细胞的凋亡情况以及Caspase-3和Survivin的表达水平。结果10～50μM的SFN能明显促进EC7906细胞凋亡，呈时间和剂量依赖性，同时，SFN还能促进Caspase-3的表达和下调Survivin水平。结论SFN可能通过促进肿瘤细胞凋亡和增加Caspase-3的水平，抑制Survivin表达而实现抗肿瘤效应的。

Effect of Sulforaphane on Esophageal Cancer Cells Apoptosis,Caspase-3 and Survivin Expression

Objective To study the effect of sulforaphane（SFN） on the apoptosis of esophageal cancer cell and the influence on expression of Caspase-3 and survivin. Methods Cultured esophageal cancer cell line EC9706,with treatment of 10～50μM SFN,the apoptosis of EC9706 and the expression level of Caspase-3 and Survivin by flow cytometry analysis. Results 10～50μM SFN could significantly promote the apoptosis of EC7906 cell in time and dose-dependent manner,meanwhile,SFN could promote the expression of Caspase-3 and downregulate Survivin level. Conclusion SFN may promote tumor cell apoptosis,increase Caspase-3 level and inhibit expression of Survivin.