| 药用峨眉野连遗传多样性的RAPD分析 |
| |
| 引用本文： | 张春平,何平,何俊星,张益锋,乔元宝,张敏,石章田,胡世俊. 药用峨眉野连遗传多样性的RAPD分析[J]. 中国中药杂志, 2010, 35(2): 138-141 |
| |
| 作者姓名： | 张春平 何平 何俊星 张益锋 乔元宝 张敏 石章田 胡世俊 |
| |
| 作者单位： | 1. 西南大学,生命科学学院,三峡库区生态环境教育部重点实验室重庆市三峡库区植物生态与资源重点实验室,重庆,400715
2. 中国科学院,昆明植物研究所,云南,昆明,650204 |
| |
| 基金项目： | 国家自然科学基金项目(30070080) |
| |
| 摘 要： | 目的:对峨眉野连进行遗传多样性研究.方法:通过RAPD技术对10个野牛峨眉野连居群共110个个体进行遗传多样性分析.结果:用14个随机引物共扩增出132条清晰条带,其中98条具多态性,平均多态性位点比率为74.24%,Nei's基因多样性指数H=0.286 3,Shannon's多样性指数I=0.362 4,遗传分化指数G_(st)=0.230 5,遗传距离和遗传一致度分别为0.193 1～0.524 5,0.501 6～0.884 3.结论:峨眉野连种水平上具有较高的遗传多样性,遗传变异主要存在于居群内部,遗传多样性与地理关系表现出明显的相关性,RAPD可以作为研究遗传多样性及遗传分化的有效标记.
|
| 关 键 词： | 峨眉野连 遗传多样性 RAPD 聚类分析 遗传分化 |
| 收稿时间： | 2009-06-12 |
RAPD analysis for genetic diversity of medicinal plant Coptis omeiensis |
| |
| ZHANG Chunping,HE Ping,HE Junxing,ZHANG Yifeng,QIAO Yuanbao,ZHANG Min,SHI Zhangtian and HU Shijun. RAPD analysis for genetic diversity of medicinal plant Coptis omeiensis[J]. China Journal of Chinese Materia Medica, 2010, 35(2): 138-141 |
| |
| Authors: | ZHANG Chunping HE Ping HE Junxing ZHANG Yifeng QIAO Yuanbao ZHANG Min SHI Zhangtian HU Shijun |
| |
| Affiliation: | School of Life Sciences, Southwest University, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Chongqing Key Laboratory of Plant Ecology and ResourcesResearch for Three Gorges Reservoir Region, Chongqing 400715, China;School of Life Sciences, Southwest University, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Chongqing Key Laboratory of Plant Ecology and ResourcesResearch for Three Gorges Reservoir Region, Chongqing 400715, China;School of Life Sciences, Southwest University, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Chongqing Key Laboratory of Plant Ecology and ResourcesResearch for Three Gorges Reservoir Region, Chongqing 400715, China;School of Life Sciences, Southwest University, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Chongqing Key Laboratory of Plant Ecology and ResourcesResearch for Three Gorges Reservoir Region, Chongqing 400715, China;School of Life Sciences, Southwest University, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Chongqing Key Laboratory of Plant Ecology and ResourcesResearch for Three Gorges Reservoir Region, Chongqing 400715, China;School of Life Sciences, Southwest University, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Chongqing Key Laboratory of Plant Ecology and ResourcesResearch for Three Gorges Reservoir Region, Chongqing 400715, China;School of Life Sciences, Southwest University, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Chongqing Key Laboratory of Plant Ecology and ResourcesResearch for Three Gorges Reservoir Region, Chongqing 400715, China;Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China |
| |
| Abstract: | Objective :To discuss the genetic diversity of Coptis omeiensis. Method : The genetic diversity of 110 individuals from 10 populations was analyzed by RAPD. Result : 14 primers were selected to produce highly reproducible RAPD bands. Among 132 amplified bands, 98 showed polymorphism, the percentage of polymorphic bands reached to 74.24%. Nei's gene diversity index (H) was 0.286 3, Shannon's information index (I) was 0.362 4, Gst was 0.230 5. The genetic distance coefficient and the similarity were 0.193 1-0.524 5 and 0.501 6-0.884 3, respectively. Conclusion : There exists a held high genetic diversity in C. omeiensis and the majority of genetic variation occurs in the populations. By cluster analysis, the geographical distribution is very obvious. The RAPD marker can be used for the analysis of the genetic diversity and genetic variation of C. omeiensis. |
| |
| Keywords: | Coptis omeiensis genetic diversity RAPD cluster analysis genetic variation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
