| 时间分辨荧光免疫法定量检测CA125试剂盒临床应用研究 |
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| 引用本文： | 梁志伟,;董志宁,;徐伟文,;李明,;吴英松. 时间分辨荧光免疫法定量检测CA125试剂盒临床应用研究[J]. 广东寄生虫学会年报, 2008, 0(11): 1119-1121 |
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| 作者姓名： | 梁志伟, 董志宁, 徐伟文, 李明, 吴英松 |
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| 作者单位： | [1]南方医科大学,广州510515; [2]广州市达瑞抗体工程技术有限公司,广州510663; [3]南方医科大学生物技术学院,广州510515 |
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| 摘 要： | 目的对自制CA125定量测定试剂盒（时间分辨荧光免疫法）进行临床应用研究，为该试剂盒的临床应用提供科学依据。方法按照自制试剂盒操作说明书对收集的410份血清进行测定，以电化学发光CA125定量测定试剂盒（Roche公司）为对照试剂，微粒子酶联免疫定量测定药盒（ABBOTT公司）为复核试剂，对测定的结果进行统计学分析。结果以电化学发光法为对照试验，其阳性符合率为98．35％，阴性符合率为98．81％，检验差异无统计学意义（P〉0．05）；相关系数为0．937，相关性良好；ROC曲线下面积为0．998。结论本试剂盒检测性能能满足临床应用的需要。
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| 关 键 词： | 癌抗原125(CA125) 时间分辨荧光免疫分析(TRFIA) 电化学发光免疫分析(ECLIA) |
Clinic Application of Time-Resolved Fluoroimmunoassay （TRFIA） Based CA125 Quantitative Diagnostic Kit |
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| Affiliation: | LIANG Zhi-wei, DONG Zhi-ning, XU Wei-wen, LI Ming, WU Ying-song(1. 2004 Grade, Major of 8-Year-Clinic Medicine, School of the First Clinical Medicine, Southern Medical University, Guangzhou 510515; 2. Guangzhou Darui Antibody CO., LTD., Guangzhou 510663; 3. School of Biotechnology, Southern Medical University, Guangzhou 510515, China) |
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| Abstract: | Objective To evaluate the applicability of CA125 quantitative diagnostic kit. Methods The levels of CA125 from 410 blood samples were detected using the CA125-TRFIA. The results were compared with ECLIA. All the procedures were performed according to the quantitative diagnositic kit instruction manual. Results The positive accordant ratio was 98.35%, and the negative accordant ratio was 98.81%. In addition, the accordance coefficient was 0.937 and the AUCROC was 0.998. The result between the two tests was not significantly different （P〉 0.05）. Conclusion Our results show that CA125-TRFIA is a valuable diagnostic kit for clinic application. |
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| Keywords: | CA125 TRFIA ECLIA |
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