华支睾吸虫重组蛋白应用于ABC-ELISA检测特异性循环抗体的研究

目的 评价重组蛋白在华支睾吸虫病诊断上的价值，探索以重组蛋白替代天然抗原用于ELISA等血清学方法诊断华支睾吸虫感染的可能性。方法 分别使用重组华支睾吸虫半胱氨酸蛋白酶(CysB)与华支睾吸虫成虫可溶性抗原(CAA)，以ABC-ELISA检测华支睾吸虫病、其它寄生虫感染及健康人血清特异性IgG．比较两种抗原用于检测的敏感性与特异性。结果 共检测了112份华支睾吸虫感染血清，使用重组抗原CysB与成虫粗抗原CAA的阳性检出率分别为92．9％(104／112)及94．6％(106／112)；分别检测健康人血清56例、日本血吸虫病血清20例及肺吸虫病血清10例．对于两种抗原，华支睾吸虫特异性IgG检测阴性率均相同．上述三类血清的阴性率分别为96．5％(54／56)、95％(19／20)及90％(9／10)。结论 华支睾吸虫重组蛋白CysB用于血清特异性抗体检测具有较高的敏感性及特异性，与成虫可溶性抗原(CAA)的诊断应用价值相近，有望成为应用于华支睾吸虫病诊断的替代抗原之一。

Study on the Application of Clonorchis sinensis Recombinant Antigen to the Detection of Specific Circulatory Antibody by ABC-ELISA

Objective Evaluating the value of recombinant proteins for the diagnosis of clonorchiasis and exploring the possibility that recombinant proteins, substituting the natural antigen, are used to the serological diagnosis of Clonorchis sinensis infection.Methods Recombinant C.sinensis cysteine proteinase(CysB) and C.sinensis adultworm antigen (CAA) were used to detect clonorchiasis by ABC ELISA. Serum samples from other parasitic infections and healthy individuals were used as control. The sensitivity and specificity of the two kinds of antigens used for the detection were compared. Results 112 cases of C.sinensis infected sera were analyzed, with a positive detection rate of 92.9% (104/112) for the recombinant antigen (CysB) and 94.6% (106/112) for CAA; 56 cases of healthy individual sera, 20 cases of schistosomiasis sera and 10 cases of paragonimiasis sera were also analyzed. All these three kinds of sera showed negative results in the detection using either CysB or CAA. The negative rates for the healthy sera, schistosomiasis sera, and paragonimiasis sera were 96.5% (54/56), 95% (19/20) and 90% (9/10), respectively. Conclusion Recombinant C.sinensis protein CysB has high sensitivity and specificity for the detection of serum specific antibody, that are comparable to CAA for diagnosis. The recombinant protein CysB is a promising alternative antigen useful for the diagnosis of clonorchiasis.