三种兔肾VX2肿瘤模型制作方法的比较

目的　探讨三种建立兔肾 VX2肿瘤模型方法的有效性。方法　研究对象为健康家兔 36只 ,动物模型建立采用三种方法 :1超声引导下肿瘤组织混悬液注射法 ;2手术直视下肿瘤组织块包埋 ;3手术直视下肿瘤组织混悬液注射法。结果　健康成年家兔 36只 ,成功建立 VX2肿瘤模型共 2 6只家兔。其中采用超声引导下肿瘤组织混悬液注射法接种 2 2只家兔的 2 8个肾脏 ,其中 10只家兔接种成功 ,成功率为 (10 /2 8) 35 .71% ;手术直视下肿瘤组织块包埋法接种 12只家兔 ,接种成功 4个左肾 ,成功率 (4 /12 ) 33.33% ;手术直视下肿瘤组织混悬液注射法接种 14只家兔左肾 ,成功 12只 ,成功率 (12 /14 ) 85 .71%。结论　三种建立兔肾 VX2肿瘤模型的方法是可行的 ,其中手术直视下肾内 VX2肿瘤组织混悬液注射法建立兔肾 VX2肿瘤模型的成功率较其他两种方法高

Comparison of three methods used for implantation of renal VX2 carcinoma

Objective To compare three methods of establishing renal VX2 carcinoma model. Methods Thirty-six healthy rabbits were used in this study, and VX2 carcinoma was successfully transplanted in 26 kidneys. Successful transplantation was done by three methods: (1) VX2 carcinoma was cut into tiny pieces in saline to make suspension of tumor tissues and the suspension was then injected into renal cortex guided by ultrasound. (2) Rabbits were operated and VX2 carcinoma was implanted into the kidney. (3) Kidneys were exposed under surgery and the suspension was injected into renal cortex. Results Animal models of renal VX2 carcinoma were successfully established using the three methods. The rates of success of the three methods were (10/28)35.71%,(4/12)33.33% and (12/14)85.71%, respectively. Conclusion All the three methods are feasible in VX2 tumor implantation. However the third method, in which the VX2 suspension is injected into renal cortex under surgical exposure, has a higher successful rate.