大孔吸附树脂纯化蒺藜呋甾皂苷和总黄酮工艺研究 |
| |
引用本文: | 刘李梅,;杨明,;郭志烨,;梅明,;韩丽,;邹文铨. 大孔吸附树脂纯化蒺藜呋甾皂苷和总黄酮工艺研究[J]. 亚太传统医药, 2014, 0(17): 35-38 |
| |
作者姓名: | 刘李梅, 杨明, 郭志烨, 梅明, 韩丽, 邹文铨 |
| |
作者单位: | [1]成都中医药大学,四川成都611137; [2]江西中医药大学,江西南昌330004; [3]四川大学,四川成都610064 |
| |
摘 要: | 目的:研究大孔吸附树脂纯化蒺藜呋甾皂苷、总黄酮的工艺条件.方法:以蒺藜呋甾皂苷、总黄酮含量及其转移率为指标,采用单因素试验法,优选树脂富集纯化蒺藜呋甾皂苷和总黄酮的最佳工艺.结果:纯化最优工艺条件:上样量为0.4g/mL,树脂径高比为1∶8,4BV水以2mL/min流速洗脱,分别用5BV50%乙醇、2BV/h 70%乙醇洗脱.D101树脂纯化后蒺藜呋甾皂苷纯度由22.59%提高到53.54%,增加了1.37倍;总黄酮的纯度由12.46%提高到32.11%,增加了1.58倍.结论:D101大孔吸附树脂可用于蒺藜呋甾皂苷和总黄酮的纯化.
|
关 键 词: | 大孔吸附树脂 蒺藜 呋甾皂苷 总黄酮 纯化工艺 |
Purification Technology of Furostanol Saponins and Total Flavonoids from Tribulus terrestris L.by Macroporous Resin |
| |
Affiliation: | Liu Limei ,Yang Ming ,Guo Zhiye , Mei Ming ,Han Li ,Zou Wenquana (1. Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China ; 2. Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China; 3. Sichuan University, Chengdu 610064, China) |
| |
Abstract: | Objective : To discuss purification technology conditions of furostanol saponins and total flavonoids from Tribulus terres- tris L. by macroporous resin. Methods: With the the content of furostanol saponins and total flavonoids as indexes, purification technology conditions were optimized by single factor experiment. Results:Optimum purification conditions were as follow: concen- tration of sample liquid was 0.4g/ml, ratio of diameter to height of macroporous resin column was 1 : 8, them were eluted by 4BV water at 2mL/min, 5BV 50% and 70% ethanol at 2BV/h respectively. The purity of furostanol saponins reached 53.54%, which was two times higher than that before purification. And the purity of total flavonoids reached 32.11% ,which was two times higher than that before purification. Conclusion:D101 Macroporous Resin is suitable for the purification of furostanol saponins and total flavonoids from Tribulus terrestris L. |
| |
Keywords: | Macroporous Resin Tribulus terrestris L. Furostanol Saponins Total Flavonoids Purification Technology |
本文献已被 维普 等数据库收录! |
|