基质金属蛋白酶-3基因启动子5A/6A多态性与高血压病的关系

目的探讨基质金属蛋白酶-3(MMP-3)基因启动子-1171(5A/6A)多态性对兰州地区汉族人群高血压病发病的可能影响。方法采用限制性片段长度多态性分析技术,检测高血压病患者(高血压病组,104例)和正常者(对照组,102例)MMP-3基因启动子SNP-1171(5A/6A)基因型的分布。结果高血压病组与对照组MMP-3基因型分布差异无统计学意义,按性别分组比较两组男性、女性之间差异均无统计学意义(P<0.05),Logistic回归分析发现男性50岁以下病例组与对照组比较差异有统计学意义(P=0.013)。并且患高血压的危险性将增加5.97倍。不同血压分级之间基因型分布和等位基因频率比较无差异(分别为P=0.950,P=0.885)。结论MMP-3基因启动子-1171(5A/6A)多态性对高血压病发病的影响与性别年龄有关,与血压水平无关。

Association between the matrix metalloproteinase 3 promoter 5A/6A polymorphism and essential hypertension

Objective to investigate the possible impact of the polymorphism in 5A/6A MMP-3 gene promoter on the risk of essential hypertension in Han Lanzhou area population.Methods 104 hypertensive patients(hypertensive group) and 102 normal persons control were recruited.Polymerase chainreaction followed by restrictionfragment length polymorphism(PCR-RFLP) was used to detect SNP-1171(5A/6A)in MMP-3 gene promoter region.Results The distribution of MMP-3 genotype was not significantly different between hypertensive patients and controls.After dividing Study sample by gender,Distribution of MMP-3 genotype and allele frequencies also were not significantly different between hypertensive and normotensive neither males nor females(P<0.05).When Multiple logistic regression analysis was used,we found 5.97-fold increased risk for hypertension associated with genotype 6A/6A(P=0.013) in young males(<50 years) according to recessive model.No significantly differences among different blood pressure level.Conclusion These results indicate that there was gender and age-specific differences in association of MMP-3 5A/6A polymorphism with hypertension in Lanzhou Han population.No marked association could be found between 5A/6A polymorphism in MMP-3 gene and different blood pressure level(P=0.950,P=0.885).