PCR检测实验恒河猴和食蟹猴群体中幽门螺杆菌和“猕猴螺杆菌”的感染

目的调查国内实验恒河猴和食蟹猴中幽门螺杆菌和"猕猴螺杆菌"的感染情况。方法参考文献中的螺杆菌属16S rRNA和幽门螺杆菌16S rRNA的引物序列,和新设计的"猕猴螺杆菌"16S rRNA特异性引物,在人工养殖的45只成年恒河猴和90只成年食蟹猴粪便样本中,通过q PCR或常规PCR检测来初步调查这两种猕猴中两种螺杆菌的感染情况。结果在恒河猴中幽门螺杆菌和"猕猴螺杆菌"的感染率均为100%,在食蟹猴中幽门螺杆菌和"猕猴螺杆菌"的感染率分别为100%和97.8%。结论证实我国人工繁育饲养的恒河猴和食蟹猴普遍存在"猕猴螺杆菌"感染。幽门螺杆菌和"猕猴螺杆菌"几乎同时存在于所有人工繁育的实验猴个体中,可能会对这两种猕猴的健康以及相关动物实验结果的准确性存在不利影响。

PCR test of Helicobacter pylori and "Helicobacter macacae" infections in rhesus and cynomolgus monkey breading colonies

Objective To investigate the status of Helicobacter pylori and "Helicobacter macacae" infection in rhesus and cynomolgus monkeys in China. Methods With the use of 16S rRNA specific primers for Helicobacter spp and Helicobacter pylori (HP) from published literatures, and new 16S rRNA specific primers designed for "Helicobacter macacae" (HM), we investigated the infection status of these two Helicobacter spps in both of 45 rhesus and 90 cynomolgus monkeys by qPCR or conventional PCR on stool samples. Results All three primer sets for 16S rRNA exhibited excellently sensitivity and specificity. Both the infection rates of HP and HM were 100% among 45 young adult rhesus monkeys. The infection rate of HP and HM in 90 young adult cynomolgus monkeys were 100% and 97.8%, respectively. Conclusions Helicobacter pylori and "Helicobacter macacae" are present in almost every artificially bred adult rhesus and cynomolgus individuals which may adversely affect the health of laboratory monkeys and the accuracy of related animal experiments.