慢性心力衰竭大鼠肝脏组织的代谢轮廓

目的建立慢性心力衰竭大鼠肝脏组织代谢轮廓模型,从代谢角度探索慢性心衰肝脏代谢变化规律,探寻对心力衰竭分子机制及疾病诊疗具有价值的特征代谢物。方法 Wistar雄性大鼠20只,心衰组行腹主动脉缩窄手术,对照组行假手术,术后饲养24周。利用代谢组学研究平台对大鼠肝脏组织进行代谢轮廓分析,建立心衰大鼠肝脏组织正交偏最小二乘判别模型及主成分分析模型,结合SPSS 19.0软件的数据处理最终得到特征代谢物。结果成功建立了主成分分析模型及正交偏最小二乘判别模型,同时从模型中筛选并鉴定了10种在心衰组与对照组具有差异的特征代谢物,包括溶血磷脂酰胆碱、溶血磷脂酰乙醇胺,油酸,甘氨胆酸,硫酸脱氢表雄酮等物质。结论构建的代谢轮廓模型很好地拟合慢性心衰大鼠肝脏的代谢紊乱状态,筛选出的特征代谢物可为该疾病的病理生理分子机制以及该疾病诊疗等提供参考和帮助。

Metabolic profiling of liver in the rats with chronic heart failure

Objective To establish a rat model of liver metabolism profile in chronic heart failure (CHF), to explore the dynamics of liver metabolism in CHF from the point of view of metabolism, and to find the characteristic metabolites valuable for the molecular mechanism and management of CHF. Methods Twenty male Wistar rats were assigned to the CHF group to receive aortic coarctation or to the control group to receive sham surgery, and were bred for 24 weeks following surgery. The metabolic profiling of the rat liver tissues was analyzed on a metabonomics research platform. Orthogonal partial least squares-discriminant analysis (OPLS-DA) model and principal component analysis (PCA) model were established for liver tissues of the CHF rats, and the characteristic metabolites were finally derived by data processing with SPSS 19.0 software. Results The PAC and OPLS-DA models were established successfully. Ten characteristic metabolites with significant differences between the CHF and control groups, including lysophosphatidyl choline, lysophosphatidyl ethanolamine, oleic acid, glycocholic acid, and dehydroepiandrosterone sulfate, were screened and identified from the models. Conclusions The metabolic disorders in CHF rats are well fitted to the established metabolic profile models, and these identified characteristic metabolites may provide reference for the pathophysiological molecular mechanism and management, etc., of chronic heart failure.