miR-338基因簇与食管癌发病风险的病例对照研究

目的: 探讨miR-338基因簇在食管癌组织中的表达模式及其与食管癌发病风险的关系。方法: 选取86例经病理确诊的食管癌患者,分别提取食管癌和癌旁组织总RNA,采用实时荧光定量PCR法检测hsa-miR-338-3p、hsa-miR-338-5p与hsa-miR-657的表达,应用配对样本t检验分析癌和癌旁组织中miRNA表达差异,Pearson相关分析检验基因簇各miRNA表达的相关性,logistic回归分析miRNA异常表达对食管癌发病风险的影响。结果: hsa-miR-338-3p与hsa-miR-338-5p在食管癌组织中的表达均较癌旁组织降低(P均<0.05),hsa-miR-657在食管癌和癌旁组织中表达的差异无统计学意义(P>0.05),hsa-miR-338-3p与hsa-miR-338-5p表达显著相关(r=0.754,P<0.05),hsa-miR-338-5p的低表达增加了食管癌的发病风险(OR=1.264,P<0.05),可能是食管癌的危险因素。结论: miR-338基因簇可能抑制了食管癌的发生,hsa-miR-338-5p可能成为食管癌诊断的潜在标志物。

A case-control study on the risk of esophageal carcinoma and miR-338 cluster

OBJECTIVE: To investigate the expression level of miR-338 cluster in esophageal carcinoma and identify the relationship between miR-338 cluster and esophageal carcinoma. METHODS: A total of 86 cases of patients with confirmed esophageal carcinoma were selected, real time RT-PCR were performed to detect the expression of hsa-miR-338-3p, hsa-miR-338-5p and hsa-miR-657. We used paired t-test to analyze the expression of miRNA in tumor and adjacent non-tumor tissues, Pearson correlation analysis to examine the correlation of the expression of each miRNA and logistic regression analysis to analyze the effect of abnormal expression of miRNAs on the risk of esophageal cancer. RESULTS: hsa-miR-338-3p and hsa-miR-338-5p were downregulated in tumor tissues compared with adjacent non-tumor tissues (P<0.05), and no statical difference was found of the expression of hsa-miR-657 between the two tissues (P>0.05). The expression of hsa-miR-338-3p and hsa-miR-338-5p were significantly correlated (r=0.754, P<0.05), and low expression of hsa-miR-338-5p was the risk factor for esophageal carcinoma (OR=1.264, P<0.05). CONCLUSION: miR-338 cluster may play an important role in the formation process of esophageal carcinoma. In addition hsa-miR-338-5p may represent the miR-338 cluster to be a useful biomarker for diagnosis.