不同剂量野百合碱联合异丙肾上腺素对大鼠血流动力学变化及心指数、右心肥厚指数研究

目的研究不同剂量野百合碱联合异丙肾上腺素对大鼠血流动力学变化及心指数、右心肥厚指数。方法采用SD大鼠64只,雌雄各半,体重200~250 g,随机分为空白对照组(n=16);模型组(n=48)。模型组分为高剂量组(n=16):80 mg/kg野百合碱采取腹腔一次性注射;10 mg/kg异丙肾上腺素采取腹腔连续注射1周;中剂量组(n=16):55 mg/kg野百合碱采取腹腔一次性注射;8 mg/kg异丙肾上腺素采取腹腔连续注射1周;低剂量组(n=16):30 mg/kg野百合碱采取腹腔一次性注射,3 mg/kg异丙肾上腺素采取腹腔连续注射1周。动物饲养到第6周后,行肺动脉压和右心室内压检测,心系数和右心室肥厚指数测定。结果与对照组比较,低剂量平均肺动脉压和右室收缩压均无差异变化;中剂量组平均肺动脉压、右室收缩压均有差异变化(P0.05),低剂量组心系数和右心室肥厚指数均无差异显著。中剂量组心系数差异极显著(P0.01),右心室肥厚指数差异显著(P0.05)。结论说明采用一次性注射野百合碱55 mg/kg,联合连续注射8 mg/kg异丙肾上腺素1周。既保证了大鼠的存活率,又保证了肺动脉高压的成功形成,野百合碱和异丙肾上腺素能对大鼠产生肺动脉高压,导致心气虚弱。

Effect of different doses of monocrotaline in combination with isopropylarterenol on the hemodynamic response, heart index and right heart hypertrophy index in rats

Objective To study the effect of different doses of monocrotaline in combination with isopropylarterenol on the hemodynamic resonse, heart index and right heart hypertrophy index in rats. Methods Sixty-four healthy adult SD rats, male:female=1:1, body weight 200-250 g, were randomly divided into blank control group (n=16) and three model groups (n=16 in each group). The high dose model group (n=16) received i.p. injection of monocrotaline 80 mg/kg once and epinephrine 10 mg/kg once daily for one week. The moderate dose group received i.p. injection of monocrotaline 55 mg/kg once and epinephrine 8 mg/kg once daily for one week. The low dose group received i.p. injection of monocrotaline 30 mg/kg once and epinephrine 3 mg/kg once daily for one week. The rats were fed for 6 weeks, and then pulmonary artery pressure and right ventricular pressure were tested and heart index and right ventricular hypertrophy index were determined. Results Compared with the control group, the mean pulmonary artery pressure and right ventricular systolic blood pressure in the low dose monocrotaline group were not significantly changed, but significantly changed in the moderate dose monocrotaline group (P<0.05). The heart index and right ventricular hypertrophy index in the low dose monocrotaline group were not significantly changed, but in the moderate dose monocrotaline group, the heart index was significantly reduced (P<0.01) and the right ventricular hypertrophy index was significantly increased (P<0.05). Conclusions The use of a single injection of 55 mg/kg monocrotaline in combination with continuous injection of 8 mg/kg isopropylarterenol once daily for one week can ensure the survival rate of rats, and the successful formation of pulmonary artery hypertension, leading to heart weakness.