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Missense exonic mitochondrial mutation in cytochrome b gene of Saccharomyces cerevisiae,resulting in core protein deficiency in complex III of the respiratory chain
Authors:Paule Chevillotte-Brivet  Gabrielle Salou  Danielle Meunier-Lemesle
Institution:(1) Laboratoire de Chimie Bactérienne du Centre National de la Recherche Scientifique, B.P. 71, F-13277 Marseille Cedex 9, France
Abstract:Summary The level of core protein I and subunit VI of mitochondrial complex III (which are coded by the nuclear genome) was found to be greatly diminished in a yeast strain carrying a mutation (W7) in the mitochondrial gene coding for cytochrome b. This suggests that intricate interactions occur in complex III biogenesis between proteins of cytoplasmic and mitochondrial origin. This mutant was characterized by a low cytochrome b level and a loss of activity in the b-c1 segment of the respiratory chain. It was compared to another mutant showing similar biochemical characteristics, but which had integrated core protein I, as shown by antibody binding experiments. In mutant devoid of core protein I, cytochrome b was found to be reducible by NADH but not by succinate, suggesting two different electron transfer pathways inside comples III from each substrate to cytochrome b heme(s).Abbreviations rho° cytoplasmic petite, with all mitochondrial DNA deleted - EPR electron paramagnetic resonance - DNFB 2,4-dinitrofluorobenzene
Keywords:Cytochrome b  Mitochondrial mutants  Core protein I  Complex III alteration
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