CD44和nm23-H1基因蛋白在喉癌中的表达

目的:探讨CD44和nm23-H1基因蛋白在喉癌中的表达及临床相关性问题.方法:应用免疫组织化学方法对组织中CD44和nm23-H1基因蛋白的表达进行观察,其中喉癌40例、喉癌旁组织20例、喉正常黏膜12例.同时与临床相关资料进行对比研究.结果:喉癌组织中CD44和nm23-H1基因蛋白的表达明显高于喉正常黏膜.喉癌有淋巴结转移者CD44基因蛋白表达高于无淋巴结转移者,有淋巴结转移者nm23-H1基因蛋白表达低于无淋巴结转移者.CD44基因蛋白表达与喉癌淋巴结转移、临床分期和病理学分级呈正相关,与肿瘤T分级无关;nm23-H1基因蛋白表达与喉癌淋巴结转移及临床分期呈负相关,与T分级无关.结论:CD44和nm23-H1基因蛋白在喉癌的发生、发展及转移过程中起着协同、调控作用,有可能成为临床判断和评价预后的重要分子生物学指标.

Expression and implication of CD44 and nm23-H1 proteins in laryngeal carcinoma

Objective:To sdudy the expression of CD44 and nm23-H1 gene proteins and their clinical significance in laryngeal carcinoma.Method:The expression of CD44 and nm23-H1 proteins were detected by immunohistochemistry method in 40 cases with laryngeal carcinoma,20 adjacent carcinoma tissues and 12 cases normal laryngeal mucosa tissues.Result:The expression of CD44 and nm23-H1 proteins in laryngeal carcinoma were much higher than that in normal laryngeal mucosa.The expression of CD44 protein in laryngeal carcinoma with metastatic lymph node was higher than that in laryngeal carcinoma without metastatic lymph node,but nm23-H1 protein lower.The expression of CD44 protein was positively correlated with the metastasis,clinnical staging and pathological classification but not correlated with T classification of laryngeal carcinoma.The expression of nm23-H1 protein was negative correlation with the metastasis and clinical staging of laryngeal carcinoma.Conclusion:CD44 and nm23-H1 gene proteins play an important coordinated regulation role in the carcinogenesis,development and metastasis of laryngeal carcinoma and will probably become the key biological marks in the judging and evaluating prognosis of laryngeal carcinoma.