不同地区间日疟原虫裂殖子表面蛋白-1基因分型及序列分析 |
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引用本文: | 肖方震,张山鹰,许龙善,黄江宏,谢汉国. 不同地区间日疟原虫裂殖子表面蛋白-1基因分型及序列分析[J]. 海峡预防医学杂志, 2010, 16(5): 3-6 |
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作者姓名: | 肖方震 张山鹰 许龙善 黄江宏 谢汉国 |
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作者单位: | 福建省疾病预防控制中心,福州350001 |
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基金项目: | 福建省自然科学基金计划项目 |
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摘 要: | [目的]分析不同地区间日疟原虫裂殖子表面蛋白-1(PvMSP-1)基因多态性。[方法]用套式PCR扩增PvMSP-1基因,纯化测序,序列分析。[结果]45个间日疟原虫样本共扩增出470 bp片段11个,400 bp片段32个,混合型2个。13个测序样本包括了Sal-1和重组型,其中重组型占69.2%(9/13)。[结论]PvMSP-1存在广泛多态性。
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关 键 词: | 间日疟原虫 裂殖子表面蛋白1 多态性 |
Genotyping and Sequence Analyzing of Plasmodium Vivax Merozoite Surface Protein-1 |
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Affiliation: | XIAO Fang-zhen,ZHANG shan-Ying,XU Long-shan,et al.(Fujian Center for Disease Control and Prevention,Fuzhou 350001 China) |
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Abstract: | [Objective] To study the polymorphism of plasmodium vivax merozoite surface protein 1(PvMSP-1) genotypes.[Methods] Nested PCR was used to amplify PvMSP-1,the PCR product of PvMSP-1 was purified and sequenced,and the sequences were analyzed.[Results] In 45 isolates,two kinds of DNA fragment of 470 bp and 400 bp were produced respectively in 11 and 32 isolates,with 2 mixture infections.Totally 13 sequenced isolates revealed two genotypes among these isolates,namely,Sal-l and recombinant types,the rate of recombinant type was 69.2%(9/13) in sequenced isolates.[Conclusions] There exist highly polymorphisms for PvMSP-1. |
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Keywords: | Plasmodium Vivax Merozoite Surface Protein-1 Polymorphism |
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